User:Aminata M. Jamina/Notebook/Biology 210 at AU

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January 23 2014 able to successfully enter text, and set up username.

January 29 2014 What is the significance of cell specialization across these three genera? -The specialization across organisms better suites them for their environment in order to promote evolution in their species.

Does evolution always move towards increased complexity? Provide an example to support your answer? -In terms of microscopic specialization, evolution has moved towards increased complexities. For example, some prokaryotes, bacteria especially have developed from unicellular to multicultural. This is obviously for survival purposes. Evolution is seems always favors more complexity, especially when an organism life and survival is at stake.

 Describe the general characteristics of the transect: location, topography, etc. - Our transect was community garden. It is located by the basketball court on the north side of campus. It consist of a variety of gardens, each designated to harvest one crop. For example, one garden is growing strawberries, while another plants cucumbers, etc.

 List the abiotic and biotic components of the transect. Abiotic Factors -wood, wired fence, rocks, tire, poles Biotic Factors -Wood chips, soil, plant roots, fruit, plant seeds A.J

January 29 2014 Group Transect:Bottom Layer,aqueous middle layer, top film

Top Layer: two thick layers that make up the top layer (one is darker than the other), fuzzy extensions at the bottom portion of the layer

Bacteria:
 -Bursana Trancatella 25um, Didinuium 57.5um, dead Paramecium, Bursaria 17.5um, Chlamydomonas 45um

Aqueous Middle Layer: brownish-yellow water

Bacteria:
 -dead paramecium aurella

Bottom layer: sand/dirt composite, two layers make up the bottom layer (the top layer consist of a thin layer of sand, the bottom layer consist of dirt), two stems growing upward (one is short, the other is really long and appears to be a root)

Bacteria:
 -Paramecium, Bursaria, 2 Gonium, Pernaema, Afflugia

If the hay infusion culture had been observed for another two months what changes would you predict to occur?

-There would probably be a larger number of bacteria species, and new and different types of bacteria will also arise. I would also expect the growth of plants to be present

What selective pressures affected the compositions of your samples?

-

A.J

March 9th, 2014 The purpose of this experiment is to observe the effects of nicotine on zebra fish development. The control group was kept in the fresh deer park water, one one may find in the natural habitat of zebra fish, while the dependent group was treated with 25ml of nicotine. Observe zebrafish embryos carefully and determine their developmental stage. 2. Set up the control group (water treated) and the test group (nicotine treated) in covered petri dishes. 3. Use 20 mls of Deerpark water and 20 healthy translucent embryos per dish. Use a dropper to transfer the eggs to the dishes with the appropriate water. 4. Count the number: of dead eggs, living embryos still in egg cases, number of living hatchlings (larvae), number of dead hatchlings. The results of the fish after day 12 and day 14 are listed below: DAY 12: All fishes were still alive, minus one which died and was removed from the rest of the fishes treated with nicotine Day 14: All wishes were dead. Day 9: There was a total of 17 fishes, three had been fixed to observes morphological features and growth in the zebra fish.

After every five to seven days, the old nicotine was cleaned out and fresh 25ml was put in in order to keep the concentration constant, and to prevent the fish "environment" from drying out. The expected outcome of the fish are that almost all will have died, and will resemble theoretical values given (kink in their tails, big bulging black eyes, slow movement, and delayed responses)

March 17, 2014 Procedure: We “sacrificed" two of the remaining 19 fish fixing them in 0.2% tricaine solution in order to anesthetize them. 4% formaldehyde was then transferred to the .2% tricaine solution. This was done by simply using a dropper to suck up the two hatchlings into a small glass case filled 25% with .2% tricaine solution and then the later addition of 4% formaldehyde. Next week 3/6, we will observe these anesthetized fish to view its morphology in greater detail. The fixed fishes were observed under the microscope. Below are the results: Body length: 600um Eyes were big, black, and looked enlarged or swollen in comparison to the original fish, heir heads were really small in comparison to their eyes, and their spines were fully developed, and they developed properly (with no kinks) despite what was predicted, extremely slow movement and delayed responses to the to water movement.

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