User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/07

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Contents

Ethanol Post-PCR Clean Up

Procedure

1) For each 10 ul of PCR product, prepare the following mixture in strip-cap tube. 1 ul of 3M sodium acetate (NaOAc) pH 5.2 25 ul chilled absolute ethanol. 2) Transfer the PCR product (20ul) into the tube containing the mixture. 3) Vortex the tube and then store it at -20 for 30-50 min. 4) Spin for 30 min at maximum speed 14.000 rpm. 5) Rremove the supernatant carefully and don't disturb the pellet. 6) Wash the pellet with 70% ethanol. And spin the tube at maximum speed for 5 min. 7) Remove the supernatant and then dry the pellet – air dry. 8) Resuspend the pellet in 10 ul in sterile water.

LCH/HCO Products

image:07April10 Plate.jpg

Sample ID Nucleic Acid Conc. Unit A260 A280 260/280 260/230 Sample Type
lco/hco 129.3ng/µl0.5860.3941.490.88DNA
lco/hco 9101.6ng/µl2.0311.2151.672.16DNA
lco/hco 1768ng/µl1.3590.8761.551.29DNA
lco/hco 2589.8ng/µl1.7971.171.541.32DNA
lco/hco 3386.2ng/µl1.7241.1441.511.28DNA
lco/hco 41169.1ng/µl3.3822.0871.621.34DNA
lco/hco 49179ng/µl3.5792.2071.621.58DNA
lco/hco 57110.9ng/µl2.2171.431.551.33DNA
lco/hco 65145ng/µl2.91.7981.611.51DNA
lco/hco 73129.3ng/µl2.5861.6191.61.45DNA

Average Concentration = 110 ng/ul

Source Plate Source Plate Well EX ID
BaltimoreA11
BaltimoreB12
BaltimoreC23
BaltimoreC34
E. WindsorA15
E. WindsorA36
E. WindsorB17
E. WindsorB28
E. WindsorC29
HarvardA210
HarvardA311
Ithaca 1A112
Ithaca 1A213
Ithaca 1A314
Ithaca 1A515
Ithaca 1A616
Ithaca 1B117
Ithaca 1B318
Ithaca 1B519
Ithaca 1C120
Ithaca 1D121
Ithaca 1D322
Ithaca 1D423
Ithaca 1D624
Ithaca 2A125
Ithaca 2A226
Ithaca 2A327
Ithaca 2A428
Ithaca 2A529
Pennsylvania (PA)B430
Tsukuba 1A431
Tsukuba 1A632
Tsukuba 1B233
Tsukuba 1B434
Tsukuba 1C135
Tsukuba 1C636
Tsukuba 3D237
Tsukuba 3D338
Tsukuba 3D439
Woods Hole 1A640
Woods Hole 1C241
Woods Hole 1C342
YokohamaB543
BaltimoreA344
BaltimoreA645
E. WindsorD446
HarvardA147
Ithaca 2A648
Pennsylvania (PA)D449
Tsukuba 1B550
YokohamaA251
BaltimoreC152
Ithaca 1A453
Pennsylvania (PA)B654
YokohamaB255
YokohamaA456
Ithaca 1C457
Ithaca 1D558
BaltimoreC559
E. WindsorA260
Pennsylvania (PA)B361
Ithaca 1C362
Ithaca 1C663
HarvardA464
Ithaca 1B465
Ithaca 1C566
BaltimoreB267
BaltimoreB368
BaltimoreB669
E. WindsorC370
Pennsylvania (PA)D671
BaltimoreB572
Ithaca 1C273
Pennsylvania (PA)D574
BaltimoreA575
Pennsylvania (PA)B176
BostonA1A
BostonA4B
Cape Cod (CC)A1C
Woods Hole 3A5D
Woods Hole 1B3E
Woods Hole 1B5F
Woods Hole 1C4G
Woods Hole 2A6H
Woods Hole 3C2I
Cape Cod (CC)A3CCA3

Alien Gene LongPCR Ethanol Cleaned

Ethanol Clean # MIX Contents ' Size Gene CCA3 Result Ethanol Cleaned ng/ul Sample Type
123AV10004 FWDAV10007 RVS8691AV212 Agood 04Mar10495.3DNA
21AV10007 FWDAV10011 RVS9262AV212 Agood 04Mar10437.4DNA
32AV10011 FWDAV10016 RVS11924AV212 Agood 02Mar10314.9DNA
424AV10016 FWDAV10020 RVS7020AV212 Agood 04Mar10408.3DNA
525AV10020 FWDAV10025 RVS11202AV212 Agood 04Mar10403.1DNA
64AV10025 FWDAV10028 RVS6843AV212 Agood 04Mar10330.8DNA
734AV10029 FWDAV10034 RVS12093AV240 Bgood 08Mar10380.5DNA
840AV10034 FWDAV10039 RVS14180AV240 Bgood 18Mar101128.6DNA
950AV10039 FWDAV10041 FWD19555AV240 Bgood 18Mar101888.1DNA
1030EU643474 FWDEU643474 RVS10062AV240 Bgood 04Mar10206DNA
1131AV10045 FWDAV10047 RVS6762AV240 Bgood 04Mar10393.3DNA
1232AV10047 FWDAV10049 RVS12719AV240 Bgood 04Mar10423.1DNA
1333AV10049 FWDAV10053 RVS9353AV240 Bgood 04Mar10364.3DNA
1478AV10091 FWDAV10092.2 RVS8127AV TEL L Agood 19Mar10515.5DNA
1585AV10092.2 FWDAV10094 RVS7036AV TEL L Agood 01Apr1353.9DNA
1616AV10094 FWDAV10098 RVS8600AV TEL L Agood 02Mar10334.4DNA
17134AV10098 FWDAV10104 FWD8000AV TEL L A&Bgood 01Apr466.4DNA
1851AV10104 RVSAV10106 RVS9618AV_TEL_L_Bgood 16Mar430.3DNA
19111AV10106 FWDAV10108.2 RVS13400AV_TEL_L_Bgood 29Mar218.4DNA
20112AV10107.1 FWDAV10108 RVS7900AV TEL L Bgood 29Mar214.1DNA
2155AV10108 FWDAV10111 RVS14846AV_TEL_L_Bgood 16Mar210.7DNA
22117AV10111 FWDAV10113 RVS8400AV TEL L Bgood 29Mar355.8DNA
23128AV10113 FWDTEL_L_B_3 RVS11500AV TEL L Bgood 01Apr422.7DNA
24131AV10114 FWDTEL_L_B_4 RVS8500AV TEL L Bgood 01Apr374.2DNA
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