User:Alexander S Mikheyev/Notebook/bee methylome/2010/04/13

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Library preparation

'
Reaction mixtureconc.CallowMature
Callow (after bisulfite conversion)??23
PCR primer PE 1.0??0.20.2
PCR primer PE 2.0??0.20.2
Phusion DNA polymerase2x55
MilliQ2.61.6
total1010
98C30 sec
98C10 sec
65C30 sec
72C30 sec
18 cycle
72C5 min

load 5.0 ul of sample

image:0074.jpg

'
Reaction mixtureconc.CallowMature
Callow (after bisulfite conversion)??23
PCR primer PE 1.0??0.20.2
PCR primer PE 2.0??0.20.2
Phusion DNA polymerase2x55
MilliQ2.61.6
total1010
98C30 sec
98C10 sec
65C30 sec
72C30 sec
22 cycle
72C5 min

load 5.0 ul of sample

image:0075.jpg



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