Turner:Solutions/Stocks

Stocks	Conc.	Units	FW (g/mol)	Vol stock (L)	g to add	Use	Notes	Last made
NaCl	5	M	58.44	0.50	146.100	Stock	autoclave	10/1/12
Tris-HCl pH 8	1	M	121.140	0.50	60.570	Tris Base	pH with ~17 mL of 12.1N HCl, autoclave	10/1/12
EDTA pH 8	0.5	M	372.240	0.50	93.060	Na Salt	pH with NaOH, autoclave	10/1/12
SDS	20	%	N/A	0.10	20.000	Stock	do not sterilize	10/1/12
Tris-HCl pH 7.4	1	M	121.140	0.50	60.570	Tris Base	pH with ~20 mL of 12.1N HCl, autoclave	10/1/12
DTT	0.1	M	154.250	0.02	0.309		f.s., 1 mL aliquots, store at -20C	10/1/12
Maltose	0.5	M	342.300	0.10	17.115	Stock	f.s.	10/1/12
HEPES pH 7.9	1	M	238.300	0.10	23.830	Stock	pH with ~3 mL 10N NaOH, f.s.	10/1/12
MgCl2	1	M	203.300	0.100	20.330	Hexahydrate	autoclave	10/1/12
MnCl2	1	M	197.910	0.100	19.791	Tetrahydrate	autoclave	10/1/12
Glycerol	50	%	N/A	N/A	N/A	Lab stock		N/A