Terrific Broth

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[edit] Summary

Terrific broth (TB, not to be confused with T Broth)

[edit] Ingredients

1. 12 g Bacto tryptone
2. 24 g Bacto yeast extract
3. 4 mL Glycerol
4. 100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, to be prepared separately from the tryptone, yeast extract, and glycerol solution.

[edit] Protocol

1. Mix tryptone, yeast extract and glycerol, and add distilled water up to 900mL
2. Pour into 2 L flask (or greater)
3. Autoclave (liquid cycle)
4. Allow liquid to cool to less than 60 degrees Centrigrade
5. Add 100 mL potassium phosphate solution