Template:SBB10 ConstructionFiles MaziarB sbb23

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 Construction of Zinc Finger Target basic part sbb23
 Wobble mb0005/mb0006           (68bp, EcoRI/BamHI)
 Sub into pBca9145-Bca1144#5    (EcoRI/BamHI, 2057+910, L)
 Product is pBca9145-sbb23      {ZFNS target}
 ----
 mb0005   Forward construction of ZFNS target basic part
 gtcgagaattccgtagatctTGTTCGGAGCCGCTTTAACCCACTC
 mb0006   Reverse construction of ZFNS target basic part
 gcgatggatccAGCACTTCCACAGAGTGGGTTAAAGCGGCTCC

JCA Notes

  • Prefix sequence should be ATG not CGT
  • Otherwise correct
  • Wiki link corrected
 Construction of Zinc Finger Target basic part sbb23
 Wobble mb0005/mb0006           (68bp, EcoRI/BamHI)
 Sub into pBca9145-Bca1144#5    (EcoRI/BamHI, 2057+910, L)
 Product is pBca9145-sbb23      {ZFNS target}
 ----
 mb0005   Forward construction of ZFNS target basic part
 gtcgagaattcATGagatctTGTTCGGAGCCGCTTTAACCCACTC
 mb0006   Reverse construction of ZFNS target basic part
 gcgatggatccAGCACTTCCACAGAGTGGGTTAAAGCGGCTCC
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