Template:SBB10 ConstructionFiles JoseG sbb38

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Biobricking of Pbad

PCR JG003-F and JG004-R on pBca1100-Bca1152.    (276 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is Pbad-pBjk2741    {Pbad-J23100}
----------------------------
JG003-F   Cloning of papC   ccataGAATTCgtccAGATCTaaccaattgtccatattgcatcag
JG004-R   Cloning of papC   ctgatGGATCCgctaatcttatggataaaaatgc

JCA Notes

  • This was a little misleading, we actually want AraC-Pbad, not just the pbad promoter itself. This construction file would adequately make a Pbad-only part
  • Nomenclature of plasmid changed
  • Modified entry on clotho
Eco/Bam transfer pBjh1601CK-ig114
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-ig114   {AraC-Pbad}
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