Template:SBB10 ConstructionFiles DorothyT sbb26

Construction File of sbb26
PCR sbb26-F and sbb26-R on pEC22-P9    (687 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1455              (EcoRI/BamHI, 2472+915, L)
Product is pBca9523-sbb26
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sbb26-F   Cloning of sbb26   ccataGAATTCatgAGATCTtgaagtgaccggattagcaacgc
sbb26-R   Cloning of sbb26   ctgatGGATCCcaatgtttttcggcagaatgcgaacgccg

JCA Notes

• Correct