From OpenWetWare
Construction of sbb05: piggieBac 5'TR
Pool BDBpb001 through BDBpb010, assemble by PCA
PCR BDBpb001/BDBpb010 on PCA reaction (344 bp, EcoRI/BamHI)
Sub in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb05
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BDBpb001 PCA assembly of sbb11 CCATAGAATTCATGAGATCTTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGC
BDBpb002 PCA assembly of sbb11 GCTATTTAGAAAGAGAGAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATC
BDBpb003 PCA assembly of sbb11 GAAATATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTCGCTGTGCATTTAGG
BDBpb004 PCA assembly of sbb11 CTCACGGGAGCTCCAAGCGGCGACTGAGATGTCCTAAATGCACAGCGACGGA
BDBpb005 PCA assembly of sbb11 CGCTTGGAGCTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTGTCACTGATTTTGA
BDBpb006 PCA assembly of sbb11 CGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATCAGTGACACTTACCGC
BDBpb007 PCA assembly of sbb11 CCGCGTGAGTCAAAATGACGCATGATTATCTTTTACGTGACTTTTAAGATTTAACTCATACG
BDBpb008 PCA assembly of sbb11 TATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGT
BDBpb009 PCA assembly of sbb11 ATATTGTTATTTCATGTTCTACTTACGTGATAACTTATTATATATATATTTTCTTGTTATAGATATCGGATC
BDBpb010 PCA assembly of sbb11 CTGATGGATCCGATATCTATAACAAGAAAATATATATATAATAA
JCA Notes
