From OpenWetWare
1) sbb12: Sleeping Beauty 3'TR
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Pool AS001-F through AS008-R, assemble by PCA
PCR AS001-F/AS008-R on PCA reaction (259 bp, EcoRI/BamHI)
Substitute in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb12
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AS001-F PCA Assembly of sbb12 CCATAGAATTCATGAGATCTTTGAGTGTATGTTAACTTCTGACCCACTGG
AS002-R PCA Assembly of sbb12 ATTTCAGCTTTTATTTCTTTCATCACATTCCCAGTGGGTCAGAAGTTAAC
AS003-F PCA Assembly of sbb12 AAAGAAATAAAAGCTGAAATGAATCATTCTCTCTACTATTATTCTGATAT
AS004-R PCA Assembly of sbb12 AGGATCACCACTTTATTTTAAGAATGTGAAATATCAGAATAATAGTAGAG
AS005-F PCA Assembly of sbb12 TAAAATAAAGTGGTGATCCTAACTGACCTTAAGACAGGGAATCTTTACTC
AS006-R PCA Assembly of sbb12 CACTTTTTCACAATTCCTGACATTTAATCCGAGTAAAGATTCCCTGTCTT
AS007-F PCA Assembly of sbb12 TCAGGAATTGTGAAAAAGTGAGTTTAATGTATTTGGCTAAGGTGTATGTA
AS008-R PCA Assembly of sbb12 CTGATGGATCCCAGTTGAAGTCGGAAGTTTACATACACCTTAGCCAAAT
JCA Notes
- correct
- Need to put parts in clotho
