Talk:CH391L/S12/Selectablegeneticmarkers

From OpenWetWare

Jump to: navigation, search


  • Jeffrey E. Barrick 10:49, 25 February 2012 (EST):Which of the commonly used antibiotic markers can E. coli mutate to be resistant to?
  • Peter Otoupal 17:24, 26 February 2012 (EST):I added an extra section on this at the end of the antibiotic section. From what I read, the common culprits are ampicillin, cefalothin, and especially tetracycline. This was for a waste-water treatment plant, which may simply be employing proportionally more of these antibiotics than others. My understanding is that the quantity of E. coli you expose to an antibiotic is the largest factor in determining the spontaneous development of antibiotic resistance, as this increases the opportunity for one particular cell to develop resistance and proliferate.
  • Jeffrey E. Barrick 10:53, 25 February 2012 (EST):Maybe provide a cross-link to the Toxin-Antitoxin page from this one in that section?
  • Peter Otoupal 16:56, 26 February 2012 (EST):Done! I also added a link to counter-selectable markers.
  • Midhat Patel 13:12, 27 February 2012 (EST): This isn't entirely relevant, but I toured a lab last year that added GFP to the gene that encoded neurons in nematodes, so that you could see the neurons themselves glowing as the worms moved around. It was really cool.
  • Peter Otoupal 14:22, 27 February 2012 (EST):That sounds totally relevant! A lot of the ideas we've been throwing around for our iGEM project involve GFP; we probably won't get to work with nematodes though.
  • Peter Otoupal 14:25, 27 February 2012 (EST):There's a neat video on youtube of a nematode wiggling around in GFP, if anyone's interested.
Personal tools