Talk:20.109(S10):Induce protein and evaluate DNA (Day5)

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Examples of induced (left) and control (right) cell pellets
Examples of induced (left) and control (right) cell pellets


Contents

TR O/N Cultures and Gels

Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group OD WT OD M124S OD Chosen mutant Pellets yellow?
Red 0.229 0.302 0.251 All bright yellow-green (after O/N). [All pellets were slightly yellow. Pellet of wildtype had more green than the others. We left them to grow overnight]
Orange 0.227 (0.1713) 0.257 (0.1823) 0.263 (0.1624) All bright yellow-green (after O/N). [Slightly green. Overnight at RT.]
Yellow 0.232 (.152) 0.244 (.151) 0.266(.146) All bright yellow-green (after O/N). [All pellets were slightly yellow, but we decided to place them in the shaker at room temp. overnight.]
Green 0.225 0.235 0.226 All bright yellow-green (after O/N).
Blue 0.231 (0.169) 0.271 (0.205) 0.218 All bright yellow-green (after O/N). [Slightly yellow except for M124S sample]
Pink 0.215 0.266 0.210 All bright yellow-green (after O/N).
Purple 0.243 (0.144) 0.237 (0.148) 0.183 (0.105) All bright yellow-green (after O/N). [Due to the imperfect (sadly) nature of our pellet, we left the samples at room temperature shaker overnight. Also, regarding our gel, we placed the 1kbp ladder in lane 8, not lane 3.]

Gels

T/R Red
T/R Red
T/R Orange
T/R Orange
T/R Yellow
T/R Yellow
T/R Green
T/R Green
T/R Blue
T/R Blue
T/R Pink
T/R Pink
T/R Purple
T/R Purple



WF O/N Cultures and Gels

Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group OD WT OD M124S OD Chosen mutant Pellets yellow?
Red 0.206 0.283 0.206 All bright yellow-green (after O/N). [Yes, except M124S is faint yellow so we decided to go overnight]
Orange 0.214 0.290 0.208 All bright yellow-green (after O/N). [yes]
Yellow 0.249 0.237 E67K2; OD = 0.232 yes; they were yellow so we decided not to go overnight.
Green 0.238 0.273 0.214 All bright yellow-green (after O/N). [NO]
Blue .281 .296 .242 Yes
Pink 0.246 0.262 0.191 The pellet in WT and E84G were bright yellow after 2 hours and 21 min. M124S was white-ish but was yellow after incubating an extra 15 minutes.
Purple .176 .192 .109 Yes

Gels

W/F Red
W/F Red
W/F Orange
W/F Orange
W/F Yellow
W/F Yellow
W/F Green
W/F Green
W/F Blue
W/F Blue
W/F Pink
W/F Pink
W/F Purple
W/F Purple



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