Talk:20.109(F13): Mod 2 Day 3 Prepare for LTS

COLD-PCR product gels

T/R Section

Lanes are loaded the same for each team HCC-827 exon 19, HCC-827 exon 21, SKOV3 exon 19, SKOV3 exon 21:

• Top gel #1:
• Lane 1: 100 bp MW ladder
• Lane 2-5: White
• Lane 6-9: Blue
• Lane 10-13: Red
• Lane 14: Aneesh

• Bottom gel #1:
• Lane 1: 100 bp MW ladder
• Lane 2-5: Orange
• Lane 6-9: Pink
• Lane 10-13: Yellow
• Lane 14: Aneesh

• Top gel #2:
• Lane 1: https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder]
• Lane 2-5: Green
• Lane 6-9: Purple
• Lane 10-13: Platinum

Note: The T/R gel was completed before PCR purification.

W/F section

Lanes are loaded the same for each team HCC-827 exon 19, SKOV3 exon 19, SKOV3 exon 21:

• Top Gel:
• Lane 1: Low Molecular Weight ladder
• Lane 2-4: White
• Lane 5-7: Blue
• Lane 8-10: Red
• Lane 11-13: Orange

• Bottom Gel:
• Lane 1: Low Molecular Weight ladder
• Lane 2-4: Pink
• Lane 5-7: Yellow
• Lane 8-10: Green
• Lane 11-13: Purple

Note: The W/F gel was completed after PCR purification.