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Mode of Action

Spectinomycin is a bacteriostatic agent that inhibits translocation of the peptidyl tRNA from the A site to the P site.

Working Concentrations and Stock Solutions

Up to 100mg/mL stock concentration in H2O. 100ug/mL final concentration.

Resistance Gene

Mutations in rpsE, the S5 protein, prevent spectinomycin from binding.

Inhibiting protein synthesis

In addition to being used for selection, spectinomycin can also be used to inhibit protein synthesis, for example, to inhibit wild-type ribosome activity in the presence of orthogonal ribosomes. 150-500μg/ml spectinomycin is commonly used for this purpose. Papers using spectinomycin in this way include -

  1. Rackham O and Chin JW. . pmid:16408021. PubMed HubMed [Rackham]
  2. Brink MF, Verbeet MP, and de Boer HA. . pmid:7758959. PubMed HubMed [Brink]
  3. Flynn JM, Neher SB, Kim YI, Sauer RT, and Baker TA. . pmid:12667450. PubMed HubMed [Flynn]
All Medline abstracts: PubMed HubMed
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