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- EcoRI/BamHI. pPint1lexAmut1 and pPint1lexAmut2 carrying mutations of the LexA box were obtained using PCR assembly (primers lexAmutL/fw, lexAmutR/pint1 and...18 KB (1,656 words) - 02:56, 25 July 2012
- Day Ligate JTK2541 into 1600 & 1601CA; Ligate LexAFull(w.t) into 9145 & 9525 Ligate both LexA(1-87)(w.t.)'s into 9525-nhe1.rbs.RFP Transform into MC1061...35 KB (58 words) - 11:02, 23 April 2012
- Plasmid Miniprep-pGREG505-lexO Enzyme cutting-pGREG505-lexO Transformation-T1743G/T1278G/Control-5μL...1 KB (0 words) - 15:15, 25 September 2017
- Plasmid Miniprep-pGREG505-lexO Enzyme cutting-pGREG505-lexO Transformation-T1743G/T1278G/Control-5μL...517 bytes (13 words) - 08:03, 18 July 2008
- engineered to overproduce LexA when infecting E. coli, which prevents the SOS response from occurring. When combined with ofloxacin, lexA phage increased killing...10 KB (1,453 words) - 20:05, 28 March 2013
- the cells. The lexA(Def) cells were GFP+ and SOS-inducible, while the lexA(Ind-) cells were SOS-uninducible and without the gfp gene. The lexA(Def) cells...7 KB (1,195 words) - 10:14, 23 January 2013
- 1128/jb.175.23.7533-7540.1993 | PubMed ID:8244922 | HubMed [OmpIgene] Sato T, Nakamura Y, Nakashima KK, Kato C, and Horikoshi K. High pressure represses...14 KB (2,381 words) - 11:06, 2 May 2013
- repair and the cancer connection. Nature Genetics 27, 247 - 254 (2001). Rich, T., Allen, R. L & Wyllie, A. H. Defying death after DNA damage. Nature 407, 777–783...10 KB (1,534 words) - 19:34, 28 January 2013
- repair and the cancer connection. Nature Genetics 27, 247 - 254 (2001). Rich, T., Allen, R. L & Wyllie, A. H. Defying death after DNA damage. Nature 407, 777–783...10 KB (1,600 words) - 19:39, 29 January 2013
- G4M1278-1&2 are right point mutations. Point mutation-T1278G/T1743G Using right wtGAL4 TG-11 Primer Name:T1743G-F & T1743G-R PCR system conditions Result: have about...2 KB (0 words) - 15:15, 25 September 2017
- mutations. Point mutation-T1278G/T1743G Using right wtGAL4 TG-11 Primer Name:T1743G-F & T1743G-R PCR system conditions Result: have about 7000 band, waiting...1 KB (59 words) - 08:00, 17 July 2008
- cannot find the band of the right size. Moreover, the size of the plasmid wasn't right, either. So they set back to the very first step, and did a lot of control...2 KB (281 words) - 08:06, 24 July 2008
- group encountered problems caused by recognition sequences on T carrier while digesting the T carrier ligation product. So they decided to use the plasmid...3 KB (383 words) - 08:05, 24 July 2008
- and DumpOnt (Ontology Visualizer). m3t4.studio Semantic Toolkit - RDF and OWL editors for the Eclipse IDE. m3t4 wiki SMORE - Create OWL Markup for HTML...28 KB (2,788 words) - 14:25, 12 January 2009
- に起こし、遺伝子発現の制御をする ２、遺伝子発現と抑制の数理モデルを作る １、i）LexA DNA binding domain－Sir2（Sir2は脱アセチル化酵素）をコードするもの（たぶんプラスミド）とii）LexA DNA binding配列をもつもの（酵母DNA）をつくる。 ii)の下流には...30 KB (5,636 words) - 10:44, 5 April 2009
- cerevisiaeにこのプラスミドを導入 ―膜を溶かす ―ガラクトースで誘導 ＊たばこ ―YqiTを再びシークエンス解析（前回読めなかったみたいなので） ―promoterをミニプレ ―制限酵素処理をして、YqiTをpromoterに入れる ―確認方法として、YqiTが臭いにおいを出すか、もしくはHisTagを付けるか。 ＊パン ―S...91 KB (22,137 words) - 21:51, 20 August 2009
- Kd_Ste12_MAPK_pT/pY/pTpY = Ste12_MAPK_pT/pY/pTpY_factor * Kd_Ste12_MAPK Kss1pT and Kss1pY bind to Ste12 with equal affinity (Ste12_Kss1_pT_factor = Ste12_Kss1_pY_factor)...42 KB (4,403 words) - 10:25, 4 October 2017
- with GST-Ste12(216-500) and GST-Ste12(450-688). LexA-Ste12 fusion proteins (LexA-Ste12(356-688) and LexA-Ste12(450-688)) lacking the activation domain (residues...10 KB (1,083 words) - 10:26, 4 October 2017
- 1T2T, 1T2W, 1T33, 1T35, 1T38, 1T39, 1T3C, 1T3D, 1T3G, 1T3I, 1T3L, 1T3N, 1T3Q, 1T3U, 1T3W, 1T47, 1T4A, 1T4B, 1T4I, 1T4L, 1T4O, 1T4X, 1T57, 1T5B, 1T5C,...96 KB (16,450 words) - 16:40, 13 May 2010
- pass filter instead of a smooth.spline function? <a href="http://t.co/c7KsVuWMwt">http://t.co/c7KsVuWMwt</a></p>— Andy (@nytowler) <a href="https://twitter...299 KB (0 words) - 23:30, 26 September 2017
