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for some aspect of a system being studied. For example, an investigator may fuse a promoter to the lacZ gene and use β-Gal levels as a readout for promoter...

especially if the BioBrick is less than 100 bp since in my experience short fragments don't fuse together as well as larger fragments. Primers for Part A (upstream...

clones are less stable when cloned and maintained in high-copy vectors, the short 2-hour induction of CopyControl BAC clones to high-copy-number does not decrease...

cross-section of the fluid channel. This would result the droplets to stop, fuse and then get released. For this process to be well-controlled, the flow rate...

proteins do. Primarily, they bind to a particular "epitope" which is usually a short peptide often from a virus. Additional proteins within the immune system...

can take an N terminus signal peptide (with HindIII site in the suffix) and fuse it behind a C terminus enzyme (with HindIII site in the prefix). Since most...

Assembly works by combining a cocktail of exonuclease, polymerase, and ligase to fuse dsDNA fragments with sufficiently (20-120 bp) homologous ends. It leaves...

genetic code! Scientists now regularly custom design and then synthesize short DNA sequence and are pioneering techniques to assemble larger custom genes...

Short Term: choose a flourescent protein (people seem opinionated on this!) and spec out the requirements to make it flouresce to aid the sensor and s...

facing) vertebral body. This section is crudely cylindrical in shape. A short, thick pedicle extends in the anterior (backwards) direction on either side...

Dr. James and collaborators who designed the first adaptamer by directly fusing together two aptamers. In principle, this adaptamer should have been able...

compaction - 2x compaction of yeost very short intergenic regions reduced number and size of introns genes shorter how does this affect how genomes work?...

amount of time to test if Nanog prevents differentiation. Is this too a short a time to see if Nanog really maintains undifferentiated cells? How did 'sonic...

it had a SpeI restriction site, thus the PCR product that we sent you is shorter. The sequence corrected is the one reported at the registry page and includes...

Two, and Author Three et al. Journal. Volume:pages. Link. Summary: Very short explanation of why this paper is relevant/ interesting. (2015) BASIC: A New...

Can Regulate Tumor Cell Metastasis. http://www.sciencemag.org/cgi/content/short/320/5876/661 The goal of this project is to create nuclear genes whose resulting...

expanded in the form of numerous invaginations which express relatively short microvilli, and a large compartment of cytoplasmic membranes, commonly called...

nucleotides in the reaction mixture. Finally, the new DNA chains made as copies fuse together as do all the DNA chains present. This represents just one cycle...

when you release vac; so, rotate/tap tube to dislodge bubbles and let them fuse then with the open atmosphere above the sample, don't worry about every one...

alternative to traditional spinal fusion. Spinal fusion is the process of fusing multiple vertebrae together in order to immobilize a vertebra that is causing...