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  • reference_seq with bowtie2. First we need to create an index of the reference sequence: $ bowtie2-build reference_seq.fa.gz bowtie_reference_seq Let's create...
    33 KB (4,704 words) - 07:18, 27 September 2017
  • # (6) seq_obj = Seq.Seq(seq,IUPAC.ambiguous_dna) seq = seq_obj.reverse_complement().tostring() # append the gene sequence self.genes.append(seq) # (7)...
    47 KB (6,829 words) - 23:04, 2 December 2008
  • from Bio import SeqIO import urllib2 as U import scipy.stats as Stat f = U.urlopen('http://www.d.umn.edu/~mhampton/HomoSmito.fa') p = SeqIO.parse(f, 'fasta')...
    12 KB (1,744 words) - 07:13, 30 September 2010
  • ecosystem with interesting ecology They are going to start doing rapid-seq on the Hi-seq machine, which will give 250PE Stephen: can we get the insert short...
    2 KB (373 words) - 12:17, 7 August 2017
  • details here [1]) compare primers sequence to the sequence I don't know the seq? e-mail sequences from day 5 that show mutation - done try to apply any of...
    6 KB (1,047 words) - 12:09, 4 June 2006
  • on the HiSeq may fail on the MiSeq. As long as your Tm matches or exceeds the Tm used for Illumina primers, the MiSeq should work, but the MiSeq’s different...
    12 KB (2,086 words) - 17:42, 13 October 2014
  • cxx file replace line 27: char *s = strchr(seq, '.'); with this one: const char *s = strchr(seq, '.'); Solved by Paolo Di Tommaso from CRG. http://grimmond...
    12 KB (1,677 words) - 02:50, 30 September 2014
  • house). Fortunately for us, the non-patterned flowcells (such as MiSeq, NextSeq and HiSeq2000) have a significantly lower issue with this than the newer instruments...
    8 KB (1,123 words) - 05:08, 28 March 2023
  • from Bio import Clustalw, GenBank, Seq from Bio.Align import AlignInfo from sets import Set from Bio.Seq import Seq,translate cline = Clustalw.MultipleAlignCL(os...
    6 KB (915 words) - 17:39, 13 March 2007
  • records = SeqIO.parse(file_handle, format="fasta") record = records.next() return record.seq.data lookup blast snp data def blast_snp_lookup(seq): result_handle...
    15 KB (2,256 words) - 01:19, 3 May 2007
  • barcode-sequencing (Bar-Seq) described by Smith et al. (Genome Res. 2009. 19: 1836-1842) on a haploid yeast knockout library. In Bar-Seq, each knockout strain...
    31 KB (4,531 words) - 14:36, 16 October 2013
  • differential expression analyses by RNA-seq. Scientific reports, 6. E14 - Cross-platform normalization of microarray and RNA-seq data for machine learning applications...
    23 KB (2,601 words) - 14:17, 22 July 2016
  • the length of the generated vector? Suggested solution: v <- c(seq(0, 60, by = 10), seq(70, 100, length.out = 11)) length(v) Suppose that you have to make...
    6 KB (918 words) - 06:51, 4 December 2008
  • UCB ig09seq/Sig/Sig040 (1) UCB ig09seq/Sig/Sig031 (1) UCB ig09seq/Sig/Sig036 (1) UCB ig09seq/Sig/Sig035 (1) UCB ig09seq/Sig/Sig033 (1) UCB ig09seq/Sig/Sig032...
    196 KB (15,771 words) - 23:00, 24 August 2010
  • records = SeqIO.parse(file_handle, format="fasta") record = records.next() return record.seq.data lookup blast snp data def blast_snp_lookup(seq): result_handle...
    22 KB (3,307 words) - 03:25, 3 May 2007
  • I clean up the header plot(a[562:2565], type="line") str(a) b<-seq(1,length(a), 2) c<-seq(2,length(a),2) plot(a[b]) plot(a[c]) aclean<-a[562:2565] head(aclean)...
    10 KB (0 words) - 23:31, 26 September 2017
  • make his/her own tools on the fly. Companies offer software packages to solve this problem, but no single software package can do anything and everything...
    7 KB (1,256 words) - 09:58, 5 June 2014
  • the end of each fragment. A computer turned that information into .abi or .seq files. Those files of the mutant sequence, along with reference files of the...
    16 KB (2,679 words) - 11:14, 18 October 2013
  • arglist ) expr return(value) if(cond) cons.expr else alt.expr for(var in seq) expr while(cond) expr repeat expr break next tapply(1:n, fac, sum) # apply...
    9 KB (922 words) - 06:27, 9 October 2009
  • gene_length, gene_strand, gene_name, locus_tag, gene_product, gene_seq, protein_seq MicrobeDB allows centralized access to the previously finished genomes...
    43 KB (5,965 words) - 14:22, 22 August 2017
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