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- use high (68oC) annealing temps don't gel purify PCR products you plan to sew Their experience lead them to believe this is a deal breaker; they rationalized...3 KB (449 words) - 21:55, 8 March 2013
- PCR Zc ABC - Zymo gel clean preparative gel fragments of PCR A, B, C sbb20 Sew - SOEing with external primers, first attempt sbb20 SOE H2O - 2nd round of...13 KB (1,519 words) - 06:58, 4 May 2010
- with "small pieces of cloth 2cm wide at separations of 4cm across the belt" sewed on, and is otherwise the same as what is described in Expts 5&6: "A rest...4 KB (837 words) - 04:44, 9 February 2010
- visible. If you have short pieces, you can sew them together with overlap extension. It is often easy to sew two pieces together if one is short (<1kb)...30 KB (4,486 words) - 20:28, 23 September 2019
- interference sequence with our own sequence using a series of primers. Then, we will sew the small pieces of DNA together in another PCR reaction. Creating Parts of...8 KB (0 words) - 02:03, 27 September 2017
