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  • Planner     OWW Basics     Resources       Glossary     Gene Mapping        RNA interference        Creating a Transgenic Organism        Assignments       ...
    2 KB (88 words) - 15:26, 28 September 2009
  • Planner     OWW Basics     Resources       Glossary     Gene Mapping        RNA interference        Creating a Transgenic Organism        Assignments       ...
    13 KB (25 words) - 18:18, 15 December 2009
  • Sample Prep Guide mRNA-Seq Directional RNA-Seq Library Prep Pre-Release DSN Normalization Sample Prep Guide mRNA-Seq Sample Prep Guide SmallRNA-Seq SmallRNAv1...
    3 KB (165 words) - 14:36, 18 September 2013
  • wild type males (double heterozygous-d u/+ +) to each of two new mating plates and mate them to L4 hermaphrodite double mutants (homozygotes d u/d u) -...
    6 KB (987 words) - 19:10, 5 October 2009
  • transformation Lab 9: Induction of bacteria for RNAi Lab 10: Scoring your worms and RNA purification Lab 11: RT PCR reactions...
    14 KB (154 words) - 16:21, 15 October 2010
  • Since homozygous mutant males usually will not mate, the desired double heterozygote is constructed by mating males that are heterozygous for your dpy mutation...
    6 KB (832 words) - 01:52, 1 October 2010
  • Since homozygous mutant males usually will not mate, the desired double heterozygote is constructed by mating males that are heterozygous for your dpy mutation...
    6 KB (858 words) - 13:29, 19 September 2011
  • program for RNA-Seq mapping. Output often processed with Cufflinks. Latest version supports TopHat detection of insertions and deletions using RNA-Seq data...
    12 KB (1,677 words) - 09:50, 30 September 2014
  • Planner     OWW Basics     Resources       Glossary     Gene Mapping        RNA interference        Creating a Transgenic Organism        Assignments       ...
    6 KB (898 words) - 18:52, 14 October 2009
  • procedure Genomic DNA prep (Qiagen) RNA prep (Trizol) ß-Gal/GUS assay LIVE/DEAD BacLight Staining Triparental Mating Transduction Chemocompetent Cells Transformation...
    3 KB (141 words) - 15:23, 19 April 2013
  • hallmark of a sucessful cross! What else can hermaphrodites do other than mate with males - might be why you have Dpys on more than one plate. If you find...
    8 KB (1,205 words) - 13:52, 18 May 2011
  • the T7 RNA polymerase gene and lacIq Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase...
    74 KB (13,034 words) - 09:11, 29 October 2024
  • Knight:RNA electrophoresis/Denaturing Knight:RNA electrophoresis Knight:RNA electrophoresis/Native Etchevers:Extraction ARN francais Sauer:RNA Purification...
    10 KB (12 words) - 01:01, 1 September 2017
  • Since homozygous mutant males usually will not mate, the desired double heterozygote is constructed by mating males heterozygous for your dpy mutation but...
    9 KB (1,423 words) - 18:46, 5 October 2010
  • Planner     OWW Basics     Resources       Glossary     Gene Mapping        RNA interference        Creating a Transgenic Organism        Assignments       ...
    8 KB (1,297 words) - 14:24, 1 September 2009
  • hallmark of a successful cross! What else can hermaphrodites do other than mate with males - might be why you have Dpys on more than one plate. If you find...
    8 KB (1,296 words) - 13:27, 12 September 2011
  • Does not carry the F plasmid F+ = Carries the F plasmid. The cell is able to mate with F- through conjugation. F′[ ] = Carries an F plasmid that has host chromosomal...
    13 KB (1,436 words) - 10:30, 7 October 2021
  • Since homozygous mutant males usually will not mate, the desired double heterozygote is constructed by mating males heterozygous for your dpy mutation but...
    9 KB (1,417 words) - 18:49, 14 October 2009
  • our unknown dpy mutation (dpy-u/+) because homozygote dumpy males do not mate properly] Available Dpy strains: The phenotype of the double heterozygote...
    11 KB (1,460 words) - 01:52, 1 October 2010
  • Abbreviations Equipment Computing Media Buffers Culturing Yeast Non-selective Mating of Yeast Sporulation in Liquid Media PCR Kinase and Anneal Oligos Restriction...
    3 KB (170 words) - 18:13, 7 March 2013
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