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buffer solution (1M) 10mL non-essential amino acids solution (10mM, 100X; Gibco catalog# 11140) 1mL 2-mercaptoethanol (1000X) sterilize using 0.2μm filter;...

at 4°C, expires 1 week) 1 X PERM/WASH (Cat. No. 554723; made from 10X stock w/ dH2O, store at 4°C, expires 1 week) PBS + 20 mM EDTA (sterile fliter, store...

biological debugger) are also needed. Current synthesis costs are about $1 per base pair. Current synthesis times for a 1,500 bp gene are of order 4 weeks...

lane (~400m read) $1,000 $1,100 $1,400 per lane 150 PE Full flowcell (2 lane, ~800m read) $2,650 $2,915 $3,710 per flowcell One lane (~400m read) $1,375...

mediμM w/ 20 μM of fMLP b. Basophil mediμM w/ 200 μg/mL milk protein c. Basophil mediμM w/ 20 μg/mL anti-IgE d. Basophil mediμM w/ 20X PMA (5 μg/mL ) e...

cell with Fox P3, prepare a Fix/Perm working solution as follow: dilute the Fix/Perm Concentrate (1 part) into the Fix/Perm Diluent (3 parts) to the desired...

cell with Fox P3, prepare a Fix/Perm working solution as follow: dilute the Fix/Perm Concentrate (1 part) into the Fix/Perm Diluent (3 parts) to the desired...

cell with Fox P3, prepare a Fix/Perm working solution as follow: dilute the Fix/Perm Concentrate (1 part) into the Fix/Perm Diluent (3 parts) to the desired...

27-32. https://dx.doi.org/10.2118/2004-062 Truby, J. M., Mueller, S. P., Llewellin, E. W., & Mader, H. M. (2014). The rheology of three-phase suspensions at...

to 100 mM. I have even used 50 mM with no detectable change.) 20 mM KCl 2 mM MgSO4 0.8 mg/mL CTAB (hexadecyltrimethylammonium bromide) 0.4 mg/mL sodium...

effectively. 150 mM NaCl 50 mM Tris-HCl pH 7.4 1% Nonidet P-40 0.25% Sodium Deoxycholate 1 mM EGTA 1mM PMSF Protease inhibitor cocktail 1 mM Na3VO4 1mM NaF Brij...

PBSMT: 2.5 mM MgCl2 3 mM KCl 0.5% Triton X-100 in PBS Add protease inhibitors PMSF: 1 mL per 100 mL buffer Use fresh PMSF: dissolve 0.035g per 1mL 100%EtOH...

cells in 100 ul of Fix/Perm solution (BD, CAT 554715) in the dark for 30 min at 4*C (fixation) b) Wash cells in 150 ul of 1X Perm/Wash buffer c) Add diluted...

kDa. 250 mM MOPS 250 mM Tris 5 mM EDTA 0.5% SDS No Need to pH. Also available commercially. 1 M sodium bisulfite add to 1X running buffer at 5 mM final concentration...

column. Generally you add a large amount of this stuff and spin the column (~750mL of PE buffer, spin for 1 min). Step 4: Remove excess EtOH. After dumping out...

mix 0.99 mL water and 0.01 mL cell suspension). Note: The desired concentration is [math]\displaystyle{ 2.5 \times 10^{11} }[/math] cells per mL, giving...

ug/ml spectinomycin (applied as an overlay), perhaps higher, I didn't test (PerM.) working concentraion for arresting protein synthesis: 150-500 ug/ml Tetracycline...

in becomes: [math]\displaystyle{ \mathrm{Re} = \frac{(1000kg/m^3)(5 m/s)(0.025 m)}{1.002 Ns/m^2} = 124.75 }[/math] This is laminar flow based on the previously...

supplemented M9 medium. 1X M9 salts 1mM thiamine hydrochloride 0.4% glycerol 0.2% casamino acids 2mM MgSO4 0.1mM CaCl2 For 1L of 1X media 200 mL 5X M9 minimal...

Rinse Buffer 10 mM Tris-Cl, pH 8.0 100 mM NaCl Lysis Buffer B-Per (Pierce) supplemented with: 5 mM EDTA (from neutral pH stock) 0.1 mg/mL Lysozyme (from...