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dissolve a measurable amount of FeCl3:7H2O and keep this as stock solution (PerM). A and B are mixed after autoclaving. Casamino acids Vary by manufacturer...

dilution: 1:100 in 1X Perm/Wash OR permeabilisation buffer). Incubate on ice, 30 minutes in the dark. Spin as in step 10. Wash twice with BD Perm/Wash OR eBioscience...

at 4°C, expires 1 week) 1 X PERM/WASH (Cat. No. 554723; made from 10X stock w/ dH2O, store at 4°C, expires 1 week) PBS + 20 mM EDTA (sterile fliter, store...

mediμM w/ 20 μM of fMLP b. Basophil mediμM w/ 200 μg/mL milk protein c. Basophil mediμM w/ 20 μg/mL anti-IgE d. Basophil mediμM w/ 20X PMA (5 μg/mL ) e...

cell with Fox P3, prepare a Fix/Perm working solution as follow: dilute the Fix/Perm Concentrate (1 part) into the Fix/Perm Diluent (3 parts) to the desired...

computed (or the software did) three different p values: per individual gene, per profile, and per Gene Ontology term. State in your own words what we need...

literacy points for the course). Rigden, D. J., Fernández-Suárez, X. M., & Galperin, M. Y. (2016). The 2016 database issue of Nucleic Acids Research and an...

to 100 mM. I have even used 50 mM with no detectable change.) 20 mM KCl 2 mM MgSO4 0.8 mg/mL CTAB (hexadecyltrimethylammonium bromide) 0.4 mg/mL sodium...

size bands. 100mL Lysis Buffer (12.5 mM Tris pH 6.8, 4%SDS) Already made; Nick has it. 1.25 mL 1M Tris (pH 8) 78.75 mL H2O Adjust pH to 6.8 if necissary...

cells in 100 ul of Fix/Perm solution (BD, CAT 554715) in the dark for 30 min at 4*C (fixation) b) Wash cells in 150 ul of 1X Perm/Wash buffer c) Add diluted...

Karina, Will, Lauren Tai, S. L., Daran-Lapujade, P., Walsh, M. C., Pronk, J. T., & Daran, J. M. (2007). Acclimation of Saccharomyces cerevisiae to low temperature:...

articles is increasing swiftly at +20% per year [5] researchers are reading more (270 articles/y) but spend less time per article (30min, down from 45min in...

PBSMT: 2.5 mM MgCl2 3 mM KCl 0.5% Triton X-100 in PBS Add protease inhibitors PMSF: 1 mL per 100 mL buffer Use fresh PMSF: dissolve 0.035g per 1mL 100%EtOH...

ug/ml spectinomycin (applied as an overlay), perhaps higher, I didn't test (PerM.) working concentraion for arresting protein synthesis: 150-500 ug/ml Tetracycline...

of a buffering agent. The purified DNA can also be eluted in TE (10 mM Tris·Cl, 1 mM EDTA, pH 8.0), but the EDTA may inhibit subsequent enzymatic reactions...

Rinse Buffer 10 mM Tris-Cl, pH 8.0 100 mM NaCl Lysis Buffer B-Per (Pierce) supplemented with: 5 mM EDTA (from neutral pH stock) 0.1 mg/mL Lysozyme (from...

we consider d[mRNA]/dt=0 and d[protein]/dt=0. Using the previously found equations, and knowing that average number of mRNA molecules per gene is 2.5 in...

250 mL broth per bottle Autoclave 250 mL dH2O * 3 g agar Autoclave To 250 mL sterile dH2O or water agar add: 2.5 mL 100X SMAJ 2.5 mL 100X MCBT 2.5 mL 30%...

0 300 μL .25 M EDTA For 75 mL T50E10: 68.25 mL ddH2O (i.e. remove 6.75 mL from 75 mL ddH2O) 3.75 mL 1 M Tris pH 8.0 3 mL .25 M EDTA 75 mL ddH2O 9.09 g...

cell with Fox P3, prepare a Fix/Perm working solution as follow: dilute the Fix/Perm Concentrate (1 part) into the Fix/Perm Diluent (3 parts) to the desired...