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4 July 2008
20:55 20.109(F08): Fall 2008 schedule (diff; hist) . . (-22) . . Nkuldell (Talk | contribs)N 20:50 20.109(F08): Module 3 oral presentations (diff; hist) . . (+4,813) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Guidelines for your 20.109 research proposal== Writing a research proposal requires that you identify an interesting topic, spend lots of time learning about it...)N 20:49 20.109(F08): ECD assembly (diff; hist) . . (+13,699) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Amazon's Kindle Epaper microcapsulesJust in time for the holiday shopping seaso...)N 20:47 20.109(F08): Phage by design, pt2 (diff; hist) . . (+9,820) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Electrodeposition is a commonly used technique in which current is applied to layer one material, usually a metal, onto another.[[Image:200px-1937...)N 20:45 20.109(F08): Phage by design (diff; hist) . . (+16,998) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== “Addition by subtraction” is a counterintuitive adage that holds true in a remarkable number of instances. Managers of sports teams have seen ...)N 20:43 20.109(F08): Transmission electron microscopy (diff; hist) . . (+6,913) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== CMSE's TEM at MIT, 13-1027 [[Image:Tem1-2.gif|thumb|right|inside a TEM, as shown at [http://nobelprize.org/educationa...)N 20:42 Talk:20.109(F08): Phage nanowires (diff; hist) . . (+476) . . Nkuldell (Talk | contribs) (New page: Sign up for time on the TEM. Please arrive in lab one hour before your time slot. Two teams can sign up for each time slot. ==T/R TEM schedule== {| border="1" |team color |TEM time bloc...)N 20:40 20.109(F08): Phage nanowires (diff; hist) . . (+13,602) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== The metal iridium has an interesting history as much for the things it hasn’t done as for the things it has. As one of the most corrosion-resist...)N 20:38 20.109(F08): Growth of phage materials (diff; hist) . . (+10,303) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Abalone shell diatoms<br> The accomplishments of the natural wo...)
19:22 20.109(F08):Module 1 (3 changes) . . (-58) . . (Page history) [Nkuldell (3×)]
N 19:18 20.109(F08):DNA engineering/FACS analysis (Day 8) (diff; hist) . . (+4,653) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== FACS stands for "Fluorescence Activated Cell Sorting." The FACS machine has revolutionized biology by allowing researchers to isolate cells based...)N 19:15 20.109(F08):DNA engineering/Lipofection (Day 7) (diff; hist) . . (+8,951) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== DNA can be put into mammalian cells in a process called transfection. If you wanted to make a mouse cell fluoresce green, you could transfect it w...)N 19:12 20.109(F08):DNA engineering/Restriction map and tissue culture (Day 6) (diff; hist) . . (+10,806) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== In the past century, we have learned a tremendous amount by studying the behavior of mammalian cells maintained in the laboratory. Tissue culture ...)N 19:11 20.109(F08):Guidelines for working in the tissue culture facility (diff; hist) . . (+6,669) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} Good cell culture technique will simultaneously protect you from anything dangerous that might be living with the cells and protect the cells from contamination by...)N 19:10 20.109(F08):DNA engineering/Examine candidate clones (Day 5) (2 changes) . . (+9,859) . . (Page history) [Nkuldell (2×)]N 19:08 20.109(F08):DNA engineering/DNA ligation and bacterial transformation (Day 4) (diff; hist) . . (+10,476) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Today you will ligate your linearized plasmid backbone with your PCR product by mixing the two in the presence of ATP and an enzyme, T4 DNA ligase...)N 19:06 Talk:20.109(F08):DNA engineering/Agarose gel electrophoresis (Day 3) (diff; hist) . . (+42) . . Nkuldell (Talk | contribs) (New page: ==Agarose Gels== ===TR lab=== ===WF lab===)N 19:04 20.109(F08): TA notes for module 1 (diff; hist) . . (+12,217) . . Nkuldell (Talk | contribs) (New page: ==General notes== '''Key preparation:''' * In case something goes wrong, spare PCR product should be prepared by TA prior to term. * A plan for preparing the high volume of cell culture ...)N 19:01 20.109(F08):DNA engineering/Agarose gel electrophoresis (Day 3) (diff; hist) . . (+10,747) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Electrophoresis is a technique that separates large molecules by size using an applied electrical field and a sieving matrix. DNA, RNA and prote...)N 18:59 20.109(F08):DNA engineering/Clean and cut DNA (Day 2) (diff; hist) . . (+10,515) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== [[Image:Be109EcoRIsite.jpg|thumb|right|200px|'''EcoRI cuts between the G and the A on each strand of DNA, leaving a single stranded DNA overhang...)N 18:57 20.109(F08):DNA engineering/DNA engineering by PCR (Day 1) (2 changes) . . (+27,738) . . (Page history) [Nkuldell (2×)] 18:56 20.109(F08):Module 2 (3 changes) . . (+200) . . (Page history) [Nkuldell (3×)]N 18:52 20.109(F08): TA's notes for module 2 (diff; hist) . . (+4,039) . . Nkuldell (Talk | contribs) (New page: =SAGA deletion Module= ==General notes== #Before module begins, streak NB312 on LB+A and miniprep (one prep/lab period). Resuspend DNA pellets in 100 ul sterile water and pool. Use this fo...) 18:39 20.109(F08) (diff; hist) . . (+31) . . Nkuldell (Talk | contribs)N 18:37 Talk:20.109(F08): Probe Western (diff; hist) . . (+51) . . Nkuldell (Talk | contribs) (New page: ==Western Results== ===TR Blots=== ===WF Blots===)N 18:36 20.109(F08): Probe Western (diff; hist) . . (+14,396) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Antibodies are useful tools in the lab. Today you will use antibodies to detect a protein on a blot. This technique, called Western analysis, can...)N 18:35 20.109(F08): Western Analysis (diff; hist) . . (+11,075) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} image credit: Justin Lo, MIT Course 20 With your molecular manipulations t...)N 18:17 Talk:20.109(F08): Microarray data analysis (diff; hist) . . (+385) . . Nkuldell (Talk | contribs) (New page: ==T/R Array data== {| border="1" |team color |team members |TAP-tag |.jpg |.txt |- |Red | | | | |- |Purple | | | | |- |Blue | | | | |- |Pink | | | | |- |Green | | | | |- |Yellow | | | |...)N 18:13 20.109(F08): Microarray data analysis (diff; hist) . . (+5,967) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Sony Playstation or Microsoft X-box? Boxers or briefs? Coke or Pepsi? We all know that taste can’t be mandated, but then how do standards arise....)N 18:12 20.109(F08): cDNA synthesis and microarray (diff; hist) . . (+17,598) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Today you will use one tool, a DNA microarray, to simultaneously examine the expression of many genes. DNA microarrays are slides with DNA sequenc...)N 18:04 20.109(F08): Screen for phenotypes, isolate RNA (diff; hist) . . (+20,978) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Life is evidently more complicated than base-pair chemistry since even a perfect cataloging of an organism’s genetic information does not allow ...)N 18:00 20.109(F08): Colony PCR (diff; hist) . . (+9,061) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Based on the numerous applications of PCR, it may seem that the technique has been around forever. In fact it is only a little more than 20 years ...)N 17:52 20.109(F08): Yeast transformation (diff; hist) . . (+19,644) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== Two toddlers, a can of white paint and a new flat screen TV...<i>"what could possibly go wrong?"</i> Microsoft Windows operating systems for web-e...)N 17:49 20.109(F08): Start-up expression engineering (diff; hist) . . (+29,632) . . Nkuldell (Talk | contribs) (New page: {{Template:20.109(F08)}} ==Introduction== From your work so far this term, you have a good understanding of (at least) two fundamental concepts. From our first experimental module it shou...)
