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  • 14:18, 13 November 2022 Js.osoriov talk contribs created page Juan Sebastian Osorio Vivas (Created page with ".")
  • 13:49, 13 November 2022 Js.osoriov talk contribs created page UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer (Created page with "For the preparation of 1 liter of potassium phosphate solution the final desired concentrations are: *0.17 Molar monobasic potassium phospate (23.135 grams/liter KH2PO4, molecular weight=136.09 grams/mole) *0.72 Molar dibasic potassium phosphate (125.410 grams/liter K2HPO4, molecular weight=174.18 grams/mole). Add the dry ingredients to a 1 liter graduated cylinder and added double distilled water until a final volume of 1 liter is reached. Sterile filter this solution...")
  • 13:46, 13 November 2022 Js.osoriov talk contribs created page UA Biophysics:Protocols:TB 1L (Created page with "'''Terrific broth (TB, not to be confused with T Broth)''' == Ingredients == #12 g yryptone. #24 g yeast extract. #4 mL Glycerol. #100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, to be prepared separately from the tryptone, yeast extract, and glycerol solution. ==Protocol== #Mix tryptone, yeast extract and glycerol, and add distilled water up to 900mL. #Pour into 1 L flask (or greater). '''Autoclave ''' #Allow liquid to cool to less than 60 degrees Centrigrade. #Add 100...")