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- 03:30, 7 April 2024 David Altman talk contribs created page File:Altman Lab Video.mov
- 03:30, 7 April 2024 David Altman talk contribs uploaded File:Altman Lab Video.mov
- 12:54, 11 January 2024 David Altman talk contribs created page File:ALTMAN CV 230111.pdf
- 12:54, 11 January 2024 David Altman talk contribs uploaded File:ALTMAN CV 230111.pdf
- 11:05, 19 September 2023 David Altman talk contribs created page File:SCRP symposium.jpg
- 11:05, 19 September 2023 David Altman talk contribs uploaded File:SCRP symposium.jpg
- 11:02, 19 September 2023 David Altman talk contribs created page File:SCRP symposium.pdf
- 11:02, 19 September 2023 David Altman talk contribs uploaded File:SCRP symposium.pdf
- 14:39, 8 September 2023 David Altman talk contribs created page File:Margaris website.jpeg
- 14:39, 8 September 2023 David Altman talk contribs uploaded File:Margaris website.jpeg
- 22:07, 5 September 2023 David Altman talk contribs created page File:Ciocanel website.jpeg
- 22:07, 5 September 2023 David Altman talk contribs uploaded File:Ciocanel website.jpeg
- 22:01, 5 September 2023 David Altman talk contribs created page File:Dawes website.jpeg
- 22:01, 5 September 2023 David Altman talk contribs uploaded File:Dawes website.jpeg
- 21:50, 5 September 2023 David Altman talk contribs created page File:Mathews website.jpeg
- 21:50, 5 September 2023 David Altman talk contribs uploaded File:Mathews website.jpeg
- 10:14, 5 September 2023 David Altman talk contribs created page File:ALTMAN CV 230905.pdf
- 10:14, 5 September 2023 David Altman talk contribs uploaded File:ALTMAN CV 230905.pdf
- 09:58, 5 September 2023 David Altman talk contribs created page File:Campi website.jpg
- 09:58, 5 September 2023 David Altman talk contribs uploaded File:Campi website.jpg
- 09:58, 5 September 2023 David Altman talk contribs created page File:Isaacson website.jpg
- 09:58, 5 September 2023 David Altman talk contribs uploaded File:Isaacson website.jpg
- 23:05, 13 June 2023 David Altman talk contribs created page File:Lablogo.jpg
- 23:05, 13 June 2023 David Altman talk contribs uploaded File:Lablogo.jpg
- 22:14, 13 June 2023 David Altman talk contribs created page Polymerizing and labeling actin (Created page with "1) Thaw out 10 uL aliquot of the 2 mg/mL G-actin stock (Cytoskeleton, Inc). * MW actin: ~43 kDa 2) Add 30 uL of G-buffer to dilute sample to 0.5 mg/mL actin (a total volume of 40 uL) 3) Let sit on ice for at least 30 minutes 4) Add 3.5 uL of polymerization salts to the 40 uL sample * Final concentration of salts in the actin: 1 mM ATP, 50 mM KCl, 2 mM MgCl2 5) Add ~0.5 uL of 300 uM stock of TRITC-phalloidin (from the -20 freezer) Recipes G-buffer: 2 mM...")
- 22:13, 13 June 2023 David Altman talk contribs created page /Polymerizing and labeling actin (Created page with "1) Thaw out 10 uL aliquot of the 2 mg/mL G-actin stock (Cytoskeleton, Inc). * (MW actin: ~43 kDa) 2) Add 30 uL of G-buffer to dilute sample to 0.5 mg/mL actin (a total volume of 40 uL) 3) Let sit on ice for at least 30 minutes 4) Add 3.5 uL of polymerization salts to the 40 uL sample * Final concentration of salts in the actin: 1 mM ATP, 50 mM KCl, 2 mM MgCl2 5) Add ~0.5 uL of 300 uM stock of TRITC-phalloidin (from the -20 freezer) Recipes G-buffer: 2 m...")
- 22:04, 13 June 2023 David Altman talk contribs created page Preparing POS fragments (Created page with " 1) Re-suspend 10 POS in 5 mL of 10 mM HEPES * We have 1 M HEPES stock (pH 7.3) * Made 10 mM stock from 1 M frozen stock: 5 mL 1 M HEPES, raise to 500 mL and sterile filter 2) Pass 30 times through 20-gauge canula – in the cold room 3) Aliquot into 100 uL stocks 4) Assess protein concentration (Bradford assay) * From communciations with Linda Bapst, they measure ~1.4 ug/uL * We measured 450-650 ug/uL (standard curve was a little noisy) * Freeze in -80 for lon...")
- 22:02, 13 June 2023 David Altman talk contribs created page /Preparing POS fragments (Created page with " 1) Re-suspend 10 POS in 5 mL of 10 mM HEPES * We have 1 M HEPES stock (pH 7.3) * Made 10 mM stock from 1 M frozen stock: 5 mL 1 M HEPES, raise to 500 mL and sterile filter 2) Pass 30 times through 20-gauge canula in the cold room 3) Aliquot into 100 uL stocks 4) Assess protein concentration (Bradford assay) * From communication with Dr. Linda Bapst, they measure 1.4 ug/uL * We measured 450-650 ug/uL (standard curve was a little noisy) * Freeze stocks of POS in...")
- 21:30, 13 June 2023 David Altman talk contribs created page File:Summer2023labpic.jpg
- 21:30, 13 June 2023 David Altman talk contribs uploaded File:Summer2023labpic.jpg
- 13:50, 13 June 2023 David Altman talk contribs created page Coating beads with photoreceptor outer segments (POS) (Created page with "In preparation for the protocol: bring the sterile 0.9% NaCl solution (which is in a large, 500 mL container in the refrigerator) into the sterile tissue culture hood, and pipet 2 mL of the solution into a 15 mL conical. This will be our non-sterile stock for the day (this is more than enough for the protocol). Return the NaCl to the refrigerator. PROTOCOL 1) Wash 35 uL of 2.0-um-diameter beads 3x in PBS. You will only need 21 uL for the POS beads; save the other cl...")
- 13:50, 13 June 2023 David Altman talk contribs created page /Coating beads with photoreceptor outer segments (POS) (Created page with "In preparation for the protocol: bring the sterile 0.9% NaCl solution (which is in a large, 500 mL container in the refrigerator) into the sterile tissue culture hood, and pipet 2 mL of the solution into a 15 mL conical. This will be our non-sterile stock for the day (this is more than enough for the protocol). Return the NaCl to the refrigerator. PROTOCOL 1) Wash 35 uL of 2.0-um-diameter beads 3x in PBS. You will only need 21 uL for the POS beads; save the other cl...")
- 13:48, 13 June 2023 David Altman talk contribs created page POS Beads (Created page with "**In preparation for the protocol: bring the sterile 0.9% NaCl solution (which is in a large, 500 mL container in the refrigerator) into the sterile tissue culture hood, and pipet 2 mL of the solution into a 15 mL conical. This will be our non-sterile stock for the day (this is more than enough for the protocol). Return the NaCl to the refrigerator. PROTOCOL 1) Wash 35 uL of 2.0-um-diameter beads 3x in PBS. You will only need 21 uL for the POS beads; save the other...")
- 13:46, 13 June 2023 David Altman talk contribs created page /POS Beads (Created page with "==Direct transfection of SF9 cells for small-scale protein purification== (Protocol is from DA, based on ZB, based on Novagen protocol) * Use 100 uL stocks of prepared POS in -80 * In preparation for the protocol: bring sterile 0.9% NaCl solution (which is in a large, 500 mL container in the refrigerator) into the sterile tissue culture hood, and pipet 2 mL of the solution into a 15 mL conical. This will be our non-sterile stock for the day (this is more than enoug...")
- 13:38, 13 June 2023 David Altman talk contribs created page Studies of ARPE-19 Phagocytosis (Created page with "='''''Studying phagocytosis in ARPE-19 cells'''''= =='''Coating beads with photoreceptor outer segments (POS)'''== </div>")
- 13:37, 13 June 2023 David Altman talk contribs created page /Studies of ARPE-19 Phagocytosis (Created page with "='''''Studies of ARPE-19 Phagocytosis'''''= =='''Making beads coated with Photoreceptor Outer Segments (POS)'''== </div>")
- 13:26, 13 June 2023 David Altman talk contribs created page File:AltmanHeadshot.jpg
- 13:26, 13 June 2023 David Altman talk contribs uploaded File:AltmanHeadshot.jpg
- 13:24, 13 June 2023 David Altman talk contribs created page File:Altman Headshot.jpg
- 13:24, 13 June 2023 David Altman talk contribs uploaded File:Altman Headshot.jpg
- 13:20, 13 June 2023 David Altman talk contribs created page File:Altman Headshot.pdf
- 13:20, 13 June 2023 David Altman talk contribs uploaded File:Altman Headshot.pdf
- 22:27, 8 June 2020 David Altman talk contribs created page File:Jung bioengineering.jpeg
- 22:27, 8 June 2020 David Altman talk contribs uploaded File:Jung bioengineering.jpeg
- 22:27, 8 June 2020 David Altman talk contribs created page File:Hickman bioengineering.jpeg
- 22:27, 8 June 2020 David Altman talk contribs uploaded File:Hickman bioengineering.jpeg
- 21:40, 7 June 2020 David Altman talk contribs created page File:ALTMAN CV 06012020.pdf
- 21:40, 7 June 2020 David Altman talk contribs uploaded File:ALTMAN CV 06012020.pdf
- 21:39, 7 June 2020 David Altman talk contribs created page File:ALTMAN CV 060120 test.pdf
- 21:39, 7 June 2020 David Altman talk contribs uploaded File:ALTMAN CV 060120 test.pdf