Smolke Endy LB Agar

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Day before, ask how much is needed to make. Usually 8 500ml bottles.

Log the batch number in your notebook.

Label each bottle with LB Agar, lot number, and your initials.

Make each 500ml in a 1 Liter bottle. Since Agar will not go into solution at room temp. It would be difficult to pour the mixture into aliquots from a larger batch.

From recipe, measure Bacto-peptone, Yeast, NaCl, and Agar into each bottle. Add 500ml of water from the millipore filter in the lab using the automatic measure.


Adhere autoclave tape. Loosely cap bottle and set in autoclave tray with 2 inches of water. Just prior to closing the door, give each bottle another swirl to ensure mixture has not settled out.

Autoclave on Liquid 500.

Individually remove each bottle and gently swirl contents. Let cool, secure cap closed and store in the weigh room cabinet.

Recipe

per 500ml bottle

  • 5.1 G Bacto Tryptone
  • 2.55 G Yeast Extract
  • 2.55 G NaCl
  • 6.1 G Agar
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