Smolke:DO Media

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Recipe

Amino Acid	Stock Concentration (g/L)	Volume Added (mL/L)	Final Concentration (mg/L)	Notes
Adenine sulfate	2	100	20	Stored at RT
Uracil	2	100	20
L-Tryptophan	10	20	20
L-Histidine hydrochloride	10	20	20
L-Arginine hydrochloride	10	20	20
L-Methionine	10	20	20
L-Tyrosine	2	150	30
L-Leucine	10	60	60
L-Isoleucine	10	30	30
L-Lysine hydrochloride	10	30	30
L-Phenylalanine	10	50	50	Stored at RT
L-Glutamic acid	10	100	100	Stored at RT
L-Aspartic acid	10	100	100	Stored at RT
L-Valine	30	50	150
L-Threonine	40	50	200
L-Serine	80	50	400
Nanopure Water		Remainder

Leave out whichever amino acid(s) you're dropping out, and replace the volume with water.
Stocks which are stored at RT are in the cabinet with autoclaved media.