Shreffler:Notebook/retinoic acid trans signaling/2009/05/20

Retinoic Acid Trans Signaling

Plasmid Purification: as per protocol

( Note: This process is used for rapid purification of transfection grade plasmid DNA)

Measure the Concentration of DNA using a UV Specrophotometer:

( Note: Info on using Specrophotometery) [1]

Sample 1x = 189.7 ng/ul ~ 171 mg of DNA Ratio = 260/280 = 1.69

Sample 2x = 112.3 ng/ul Ratio = 260/280 = 1.8

Prepare Glycerol Stock of Bacteria Culture:

1. Vortex the cell culture in 15 ml tube

2. Prepare a 20% glycerol solution (80% water + 20% glycerol)

3. Take 400 ul of glycerol solution and place it into a 1.5 ml tube)

4. Add 600 ul of the cell culture, invert mixture up and down, then place in -80 degree freezer for storage

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