Sack and Yarov-Yarovoy Labs Department of Physiology and Membrane Biology University of California, Davis
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Acidwash 03/22/11.
Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) . Cell Media with Selection Agents (Blasticidin, Zeocin) . Cell Splitting Protocol. Cell Transfection (Lipofectamine LTX). Cell Transfection (Lipofectamine). Cell Transfection (Novachoice). Cell Harvesting for Electrophysiology. Cell Plating for Electrophysiology . Freezing CHO Cells. Plating Subclones for Ephys. Plating Subclones for Fluorescent Screening. Subcloning Cells. Testing TREX Subclones Ephys. Thawing CHO cells.
Agar Bridge Formation.
Bacteria Transformation (One Shot Top Ten Invitrogen). Bacteria Transformation Invitrogen . Growth of Bacterial Cultures . Subcloning Efficiency DH5a Chemically Competent E. Coli .
Ephys Solutions 08/15/12. Example 20K External 11/08/11. Reagent Stocks Template 11/21/11. Solutions Template 11/22/11.
ELISA Protocol for a 96-well Plate. ELISA Spreadsheet Skeleton .