SEED/2008/Supplies

Consumable supplies

Item	Supplier	Product Number	Unit Price	Total Items	Total Units	Total Cost	Week 1	Week 2	Week 3	Week 4	Week 5	Week 6	Week 7	Week 8	Week 9	Week 10
"Petri Dishes, 100mm x 15mm"	VWR	25373-100	$146.90 per 500	450	0.9	146.9	350	20	0	0	80	0	0	0	0	0
Composition Book	COOP	N/A	$2.00 ea	20	20	40	20	0	0	0	0	0	0	0	0	0
"LB Broth, Miller Dehydrated 500 g"	VWR	90003-350	$52.33 per 20 L	16	0.8	52.33	9	0.5	0	0	2	1	0.5	1	1	1
Bacto Agar				168.75		0	131.25	7.5	0	0	30	0	0	0	0	0
"S-Gal/LB Agar Blend, 500 mL"	Sigma	S9938-500ML	$35.80 per 500 mL	600	1.2	71.6	100	500	0	0	0	0	0	0	0	0
Ampicillin	Sigma	A0166-5G	$56.90 per 5 g	1	0.2	56.9	0.3	0.05	0	0	0.2	0.1	0.05	0.1	0.1	0.1
Chloramphenicol	Sigma	C0378-5G	$26.60 per 5 g	0.1	0.02	26.6	0.075	0.025	0	0	0	0	0	0	0	0
Kanamycin	Sigma	60615-5G	$63.10 per 5 g	0.15	0.03	63.1	0.15	0	0	0	0	0	0	0	0	0
"Pipette Tips, 1mL"				0		0
"Pipette Tips, 200uL"				0		0
"Pipette Tips, 20uL"				0		0
"Sterile Disposible 5mL Polystyrene Pipettes, 200"	VWR	53283-706	$82.27 per 200	200	1	82.27	0	0	0	0	0	40	40	40	40	40
"Lab Markers, 10-Pack Assorted"	VWR	52877-140	$33.39 ea	2	2	66.78	2	0	0	0	0	0	0	0	0	0
"Microscope Slides, Plain, 72"	VWR	16004-430	$19.55 per 72	1	1	19.55	1	0	0	0	0	0	0	0	0	0
Microscope Coverslips	VWR	16004-094	$23.99 per Box	1	1	23.99	1	0	0	0	0	0	0	0	0	0
"Mechanical Crygenic Freezer Boxes, 12-Pack"	VWR	82021-114	$37.09 per 12	20	1.666666667	74.18	0	20	0	0	0	0	0	0	0	0
"PstI, 10000 Units"	VWR	101228-764	$69.60 ea	2000	0.2	69.9	0	1000	0	1000	0	0	0	0	0	0
"XbaI, 3000 Units"	VWR	101228-792	$75.60 ea	2000	0.666666667	75.6	0	1000	0	1000	0	0	0	0	0	0
"SpeI, 500 Units"	VWR	101228-726	$73.20 ea	400	0.8	73.2	0	200	0	200	0	0	0	0	0	0
"EcoRI, 10000 Units"	VWR	101228-592	$63.60 ea	2000	0.2	63.6	0	1000	0	1000	0	0	0	0	0	0
Agarose	Sigma	A9414-25G	$184.00 per 25 g	15	0.6	184	0	5	5	5	0	0	0	0	0	0
"1.5mL Eppendorf Tubes, 500 Pack, Clear"	VWR	21008-959	$41.00 per 500	1900	3.8	164	0	100	200	200	200	200	300	300	300	100
"TBE Buffer, 10X"	VWR	82021-496	$39.12 per L	1.8	1.8	78.24	0	0.6	0.6	0.6	0	0	0	0	0	0
"6X DNA Gel Loading Buffer, 5mL"	VWR	80030-946	$36.43 per 5mL	0.6	0.12	36.43	0	0.2	0.2	0.2	0	0	0	0	0	0
Benchtop 1 kb DNA Ladder	VWR	PAG7541	$123.00 per 600 uL	0	0	0	0				0	0	0	0	0	0
"Syber Safe DNA Gel Stain, 10000X"	Invitrogen	S33102	$45.00 per 400 uL	600	1.5	90	0	200	200	200	0	0	0	0	0	0
"Laboratory Labeling Tape - Rainbow, 1/2"", 24"	VWR	36429-206	$104.19 ea	1	1	104.19	1	0	0	0	0	0	0	0	0	0
"Razor Blades, Pack of 100"	VWR	55411-055	$22.88 per 100	20	0.2	22.88	0	0	0	20	0	0	0	0	0	0
PCR Kit				50		0	0	0	50	0	0	0	0	0	0	0
"PCR Tubes & Caps, Pack of 1000"	VWR	20170-010	$121.8 per 1000	50	0.05	121.8	0	0	50	0	0	0	0	0	0	0
"Qiagen PCR Clean-Up Kit, 50 Rxn"	Qiagen	28104	$91.00 per 50	70	1.4	182	0	0	50	20	0	0	0	0	0	0
"Qiagen Mini-Prep Kit, 50 Rxn"	Qiagen	27104	$77.00 per 50	90	1.8	154	0	0	10	0	0	80	0	0	0	0
"Qiagen Gel Extraction Kit, 50 Rxn"	Qiagen	28704	$91.00 per 50	20	0.4	91	0	0	0	0	20	0	0	0	0	0
Primers						0
3' Primer:						0
5' Primer:						0
"IPTG, 200 mM Filtered Solution"	Sigma	I1284-5ML	$38.10 per 5 mL	4	0.8	38.1	0	0	0	0	2	0	0	0	1	1
N-(3-Oxooctanoyl)-L-homoserine lactone (AHL)	Sigma	O1764-10MG	$43.80 per 10 mg	3	0.3	43.8	0	0	0	0	0	0	1	0	1	1
X-Gal	Sigma	B4252-100MG	$69.30 per 100 mg	100	1	69.3	0	0	0	0	80	0	0	0	10	10
"T4 DNA Ligase, 1500 Units"	VWR	VW85V1380	$14.21 ea	800	0.533333333	14.21	0	0	0	0	800	0	0	0	0	0
One Shot TOP10 Chemically Competent E coli	Invitrogen	C4040-06	$605.00 per 80	80	1	605	0	0	0	0	80	0	0	0	0	0
SOC Media	VWR	101384-910	$61.60 per 10x20mL	80	0.4	61.6	0	0	0	0	80	0	0	0	0	0
"BD Falcon Round Bottom Disposable Culture Tubes, 14mL"	VWR	60819-524	$134.44 per 500	0	0	0
"VWR Disposible Cuvettes, 1.5 mL"	VWR	58017-847	$81.73 per 500	500	1	81.73	0	0	0	0	0	0	100	100	200	100
Beta-Mercaptoethanol	Sigma	M6250-100mL	$20.70 per 100 mL	3	0.03	20.7	0	0	0	0	0	0	3	0	0	0
Na2CO3 (Sodium Carbonate)	Sigma	S7795-500G	$48.00 per 500 g	100	0.2	48	0	0	0	0	0	0	100	0	0	0
1% Hexadecyltrimethylammonium bromide (CTAB)	Sigma	52365-50G	$29.50 per 50 g	0.05	0.001	29.5	0	0	0	0	0	0	0.05	0	0	0
10% Sodium deoxycholate	Sigma	30970-25G	$27.50 per 25 g	0.025	0.001	27.5	0	0	0	0	0	0	0.025	0	0	0
o-nitrophenol-beta-D-Galactoside	Sigma	N1127-1G	$25.40 per 1 g	0.5	0.5	25.4	0	0	0	0	0	0	0.5	0	0	0
Gloves
Kim Wipes
Grand Total						3299.88