SEED/2008/Supplies

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Consumable supplies

Item Supplier Product Number Unit Price Total Items Total Units Total Cost Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10
"Petri Dishes, 100mm x 15mm" VWR 25373-100 $146.90 per 500 450 0.9 146.9 350 20 0 0 80 0 0 0 0 0
Composition Book COOP N/A $2.00 ea 20 20 40 20 0 0 0 0 0 0 0 0 0
"LB Broth, Miller Dehydrated 500 g" VWR 90003-350 $52.33 per 20 L 16 0.8 52.33 9 0.5 0 0 2 1 0.5 1 1 1
Bacto Agar 168.75 0 131.25 7.5 0 0 30 0 0 0 0 0
"S-Gal/LB Agar Blend, 500 mL" Sigma S9938-500ML $35.80 per 500 mL 600 1.2 71.6 100 500 0 0 0 0 0 0 0 0
Ampicillin Sigma A0166-5G $56.90 per 5 g 1 0.2 56.9 0.3 0.05 0 0 0.2 0.1 0.05 0.1 0.1 0.1
Chloramphenicol Sigma C0378-5G $26.60 per 5 g 0.1 0.02 26.6 0.075 0.025 0 0 0 0 0 0 0 0
Kanamycin Sigma 60615-5G $63.10 per 5 g 0.15 0.03 63.1 0.15 0 0 0 0 0 0 0 0 0
"Pipette Tips, 1mL" 0 0
"Pipette Tips, 200uL" 0 0
"Pipette Tips, 20uL" 0 0
"Sterile Disposible 5mL Polystyrene Pipettes, 200" VWR 53283-706 $82.27 per 200 200 1 82.27 0 0 0 0 0 40 40 40 40 40
"Lab Markers, 10-Pack Assorted" VWR 52877-140 $33.39 ea 2 2 66.78 2 0 0 0 0 0 0 0 0 0
"Microscope Slides, Plain, 72" VWR 16004-430 $19.55 per 72 1 1 19.55 1 0 0 0 0 0 0 0 0 0
Microscope Coverslips VWR 16004-094 $23.99 per Box 1 1 23.99 1 0 0 0 0 0 0 0 0 0
"Mechanical Crygenic Freezer Boxes, 12-Pack" VWR 82021-114 $37.09 per 12 20 1.666666667 74.18 0 20 0 0 0 0 0 0 0 0
"PstI, 10000 Units" VWR 101228-764 $69.60 ea 2000 0.2 69.9 0 1000 0 1000 0 0 0 0 0 0
"XbaI, 3000 Units" VWR 101228-792 $75.60 ea 2000 0.666666667 75.6 0 1000 0 1000 0 0 0 0 0 0
"SpeI, 500 Units" VWR 101228-726 $73.20 ea 400 0.8 73.2 0 200 0 200 0 0 0 0 0 0
"EcoRI, 10000 Units" VWR 101228-592 $63.60 ea 2000 0.2 63.6 0 1000 0 1000 0 0 0 0 0 0
Agarose Sigma A9414-25G $184.00 per 25 g 15 0.6 184 0 5 5 5 0 0 0 0 0 0
"1.5mL Eppendorf Tubes, 500 Pack, Clear" VWR 21008-959 $41.00 per 500 1900 3.8 164 0 100 200 200 200 200 300 300 300 100
"TBE Buffer, 10X" VWR 82021-496 $39.12 per L 1.8 1.8 78.24 0 0.6 0.6 0.6 0 0 0 0 0 0
"6X DNA Gel Loading Buffer, 5mL" VWR 80030-946 $36.43 per 5mL 0.6 0.12 36.43 0 0.2 0.2 0.2 0 0 0 0 0 0
Benchtop 1 kb DNA Ladder VWR PAG7541 $123.00 per 600 uL 0 0 0 0 0 0 0 0 0 0
"Syber Safe DNA Gel Stain, 10000X" Invitrogen S33102 $45.00 per 400 uL 600 1.5 90 0 200 200 200 0 0 0 0 0 0
"Laboratory Labeling Tape - Rainbow, 1/2"", 24" VWR 36429-206 $104.19 ea 1 1 104.19 1 0 0 0 0 0 0 0 0 0
"Razor Blades, Pack of 100" VWR 55411-055 $22.88 per 100 20 0.2 22.88 0 0 0 20 0 0 0 0 0 0
PCR Kit 50 0 0 0 50 0 0 0 0 0 0 0
"PCR Tubes & Caps, Pack of 1000" VWR 20170-010 $121.8 per 1000 50 0.05 121.8 0 0 50 0 0 0 0 0 0 0
"Qiagen PCR Clean-Up Kit, 50 Rxn" Qiagen 28104 $91.00 per 50 70 1.4 182 0 0 50 20 0 0 0 0 0 0
"Qiagen Mini-Prep Kit, 50 Rxn" Qiagen 27104 $77.00 per 50 90 1.8 154 0 0 10 0 0 80 0 0 0 0
"Qiagen Gel Extraction Kit, 50 Rxn" Qiagen 28704 $91.00 per 50 20 0.4 91 0 0 0 0 20 0 0 0 0 0
Primers 0
3' Primer: 0
5' Primer: 0
"IPTG, 200 mM Filtered Solution" Sigma I1284-5ML $38.10 per 5 mL 4 0.8 38.1 0 0 0 0 2 0 0 0 1 1
N-(3-Oxooctanoyl)-L-homoserine lactone (AHL) Sigma O1764-10MG $43.80 per 10 mg 3 0.3 43.8 0 0 0 0 0 0 1 0 1 1
X-Gal Sigma B4252-100MG $69.30 per 100 mg 100 1 69.3 0 0 0 0 80 0 0 0 10 10
"T4 DNA Ligase, 1500 Units" VWR VW85V1380 $14.21 ea 800 0.533333333 14.21 0 0 0 0 800 0 0 0 0 0
One Shot TOP10 Chemically Competent E coli Invitrogen C4040-06 $605.00 per 80 80 1 605 0 0 0 0 80 0 0 0 0 0
SOC Media VWR 101384-910 $61.60 per 10x20mL 80 0.4 61.6 0 0 0 0 80 0 0 0 0 0
"BD Falcon Round Bottom Disposable Culture Tubes, 14mL" VWR 60819-524 $134.44 per 500 0 0 0
"VWR Disposible Cuvettes, 1.5 mL" VWR 58017-847 $81.73 per 500 500 1 81.73 0 0 0 0 0 0 100 100 200 100
Beta-Mercaptoethanol Sigma M6250-100mL $20.70 per 100 mL 3 0.03 20.7 0 0 0 0 0 0 3 0 0 0
Na2CO3 (Sodium Carbonate) Sigma S7795-500G $48.00 per 500 g 100 0.2 48 0 0 0 0 0 0 100 0 0 0
1% Hexadecyltrimethylammonium bromide (CTAB) Sigma 52365-50G $29.50 per 50 g 0.05 0.001 29.5 0 0 0 0 0 0 0.05 0 0 0
10% Sodium deoxycholate Sigma 30970-25G $27.50 per 25 g 0.025 0.001 27.5 0 0 0 0 0 0 0.025 0 0 0
o-nitrophenol-beta-D-Galactoside Sigma N1127-1G $25.40 per 1 g 0.5 0.5 25.4 0 0 0 0 0 0 0.5 0 0 0
Gloves
Kim Wipes
Grand Total 3299.88
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