SBB09 Construct43

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 Construction of {<TraT>} part

 PCR Ons001F/Ons002R on TraT         (342 bp, BamHI/EcoRI)
 Digest pBca9495CA-Bca1144#5         (BamHI/EcoRI, 3039+910, L)
 Product is pBca9495-M10020          {<TraT>}
 ---------------------------------------------------
 Ons001F  Forward BglII for TraT ccataGAATTCatgAGATCTaaaccccgtaaacagcattcgcctg
 Ons002R  Reverse BamH1 for TraT ctgatGGATCCggccaaatcaatcgcaaatattctcccccccgaaCtcaggc
 JCA:  I think you got the wrong template sequence somehow here.  See revision below:
Construction of {<TraT>} part

PCR Ons001F/Ons002R on TraT         (700 bp, BamHI/EcoRI)
Digest pBca9495CA-Bca1144#5         (BamHI/EcoRI, 3039+910, L)
Product is pBca9495CA-M10020          {<TraT>}
---------------------------------------------------
Ons001F	Forward oligo for TraT	
ccataGAATTCatgAGATCTtgtggtgcgatgagcacagc
Ons002R	Reverse oligo for TraT	
ctgatGGATCCgagaatatttgcgattgatttgg
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