SBB09 Construct36

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Construction of <a~TraA> basic part

PCR Oph022-F/Oph023-R on F plasmid (398 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-M10036  {a~TraA>}
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Oph022-F        foward oligo for cloning of {a~TraA>}
ccaaaGAATTCatgAGATCTacttatgaatgctgttttaagtgttcaggg
Oph023-R        reverse oligo for removal of stop codon {a~TraA>}
gcaaaGGATCCgaggccaacgacggccatac
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