RobWarden:M2D1

Module 2, Day 1 For Next Time

Expected Cells

Hemocytometer

Count:

10				16
5				15

Average = 11.5 -> 115,000 cells/ml
Diluted by 1:100 -> 1,150 cells/ml
Plated 3 ml/well -> 3,450 cells/well

General Formula

[math]\displaystyle{ \mbox{Expected Cells} = \mbox{Number Plated} * \mbox{Plating Efficiency} * 2^{\frac{\mbox{Time Since Plating} - \mbox{Seating Time}}{\mbox{Doubling Time}}} }[/math]

My plates

• Number plated = 3,450 cells/well
• Plating efficiency = .25
• Time since plating = 48 hr
• Seating time = 24 hr
• Doubling time = 24 hr
• Expected cells = [math]\displaystyle{ 3450\ \frac{cells}{well} * .25 * 2^{\frac{48\ hr - 24\ hr}{24\ hr}} = 1725\ \frac{cells}{well} }[/math]

GFP V68L

Although this mutation doesn't look like much, it is enough to destabilize the fluorophore and make the protein fluoresce yellow instead of green.

Calcium Solution

[math]\displaystyle{ 20 \times 10^{-3}\ \frac{mol}{L} * 110.99\ \frac{g}{mol} * 50 \times 10^{-3}\ L = 0.111\ g }[/math]