RobWarden:M1D2
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Module 1, Day 2 For Next Time
Molecular Markers
Ladder Data
| Length (BP) | Log10(Length) | Distance (mm) |
|---|---|---|
| 10000 | 4.000 | 11.6 |
| 8000 | 3.903 | 12.4 |
| 6000 | 3.778 | 12.4 |
| 6000 | 3.778 | 14.0 |
| 5000 | 3.699 | 14.8 |
| 4000 | 3.602 | 16.3 |
| 3000 | 3.477 | 18.3 |
| 2000 | 3.301 | 21.4 |
| 1500 | 3.176 | 24.0 |
| 1000 | 3.000 | 27.9 |
| 500 | 2.699 | 33.6 |
- Distances measured in Photoshop
Data Analysis
[math]\displaystyle{ \operatorname{Log_{10}}{(Length)} = -0.057*Distance+4.576 }[/math]
[math]\displaystyle{ R^2 = 0.987 }[/math]
Features
| Feature | Distance | Log10(Length) | Length (BP) |
|---|---|---|---|
| Supercoiled | 10.0 | 4.006 | 10139 |
| Open Circle | 14.0 | 3.778 | 5998 |
| Digested | 12.1 | 3.886 | 7697 |
- Distances measured in Photoshop
Plaque Math
[math]\displaystyle{ 10 {\mu}L * {10^{12} \frac{\mbox{plaque forming units}}{{\mu}L}}
* {10^{-8}\ \mbox{dilution}} = 10^5\ \mbox{plaque forming units} }[/math]
DH5 does not have an F vector so it will not have an F pilus. M13 Integrates into the F pilus, so no plaques should be seen in DH5.
Revised M13K07 Map
Synthetic biology is about engineering while genetic engineering is about biology.
This is a completely valid statement. Synthetic biology embodies engineering, applying knowledge to create a new system that is advantageous to society. Genetic engineering, on the other hand, is very much a scientific method, perturbing, rearranging, or introducing material into a system to study the system's reaction.
