RobWarden:M1D2

From OpenWetWare

Jump to: navigation, search

Contents

Module 1, Day 2 For Next Time

Molecular Markers

Ladder Data

Length (BP) Log10(Length) Distance (mm)
10000 4.000 11.6
8000 3.903 12.4
6000 3.778 12.4
6000 3.778 14.0
5000 3.699 14.8
4000 3.602 16.3
3000 3.477 18.3
2000 3.301 21.4
1500 3.176 24.0
1000 3.000 27.9
500 2.699 33.6
  • Distances measured in Photoshop

Data Analysis

Image:RobWardenDigestData14Feb07.jpg
\operatorname{Log_{10}}{(Length)} = -0.057*Distance+4.576
R2 = 0.987

Features

Feature Distance Log10(Length) Length (BP)
Supercoiled 10.0 4.006 10139
Open Circle 14.0 3.778 5998
Digested 12.1 3.886 7697
  • Distances measured in Photoshop

Plaque Math

10 {\mu}L * {10^{12} \frac{\mbox{plaque forming units}}{{\mu}L}}
* {10^{-8}\ \mbox{dilution}} = 10^5\ \mbox{plaque forming units}

DH5 does not have an F vector so it will not have an F pilus. M13 Integrates into the F pilus, so no plaques should be seen in DH5.

Revised M13K07 Map

Image:RobWardenM13K07Plasmid.jpg

Synthetic biology is about engineering while genetic engineering is about biology.

This is a completely valid statement. Synthetic biology embodies engineering, applying knowledge to create a new system that is advantageous to society. Genetic engineering, on the other hand, is very much a scientific method, perturbing, rearranging, or introducing material into a system to study the system's reaction.

Personal tools