Registry/Measurement kit/Notebook/2007-6-26

From OpenWetWare

Jump to: navigation, search

Contents

To do

  1. Gradient scarring PCR on E0240 (and I2055) and run gel (done)
  2. Spin down device cultures/replate (done)
  3. Run gel on PCR product from yesterday (done)
  4. Cleanup yesterday's digests and PCR products (done. in Measurement Kit box)
  5. Make new Tet plates (done)

Preparation of 3K3

  • Mini-prepped remaining 4mL of 3K3 (glycerol) culture
    • Concentration was 44.1 ng/µL
  • Digest of 3K3 running overnight.
  • (23µL DNA + 19.5µL H2O)

Analysis of PCR of I2055

  • Gel had no band.
  • Will do gradient with E0240

RFP Device cultures

  • RBS tester had one colony; others had no growth.
  • made overnight culture to look at tomorrow

Gradient PCR

  • Running gel with 2 20-lane rows:
  1. E0240 _ L _ _ 1 2 3 ... _ L _ _
  2. I2055   _ _ L _ 1 2 3 ... _ _ L _
Personal tools