Registry/Measurement kit/Notebook/2007-11-9

--Adam J. Rubin 16:20, 12 November 2007 (CST) Diluted 1:1000 (5ul in 5 ml M9 media) in the morning after overnight growth. Pre-warmed the M9 media before to counter temperature shock.

Noticed a difference in color. After 4.5 hours tubes 4 and 14 were noticeably more clear than all others. Made plate per standard procedure. [1]

Plate layout:

1. I20244 w/ J23116 (w) (GFP , E/S)	x2	x3	9. I20245 w/ J23116 (w) (GFP, E/X)	x2	x3
2. I20244 w/ R0040 (s) (GFP , E/S)	x2	x3	10. I20245 w/ R0040 (s) (GFP, E/X)	x2	x3
3. I20246 w/ J23116 (w) (RFP, E/S)	x2	x3	11. I20247 w/ J23116 (w) (RFP, E/X)	x2	x3
4. I20246 w/ R0040 (s) (RFP, E/S)	x2	x3	12. I20247 w/ R0040 (s) (RFP, E/X)	x2	x3
5. I20248 w/ B0031 (w) (GFP, E/X)	x2	x3	13. I20249 w/ B0031 (w) (GFP, S/X)	x2	x3
6. I20248 w/ B0032 (s) (GFP, E/X)	x2	x3	14. I20251 w/ B0032 (s) (RFP, S/X)	x2	x3
7. I20250 w/ B0031 (w) (RFP, E/X)	x2	x3	15. blank M9 media	x2	x3
8. I20250 w/ B0032 (s) (RFP, E/X)	x2	x3	16. empty TOP10 cells	x2	x3