Quenching Buffer

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Quenching Buffer

Recipe

1M Tris HCl (pH 7.5)

How to make it

  • Weigh out 15.760 g Tris HCl (MW: 157.60 g/mol) and add to a 100 mL beaker.
  • Add H20 to the beaker.
  • Add a magnetic flea and place on a magnetic stirring plate to mix the solution.
  • Add a pH meter into the solution to observe the pH.