Quenching Buffer
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Quenching Buffer
Recipe
1M Tris HCl (pH 7.5)
How to make it
- Weigh out 15.760 g Tris HCl (MW: 157.60 g/mol) and add to a 100 mL beaker.
- Add H20 to the beaker.
- Add a magnetic flea and place on a magnetic stirring plate to mix the solution.
- Add a pH meter into the solution to observe the pH.