Promega Quick Ligation

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1.CIP treatment

DNA: 15μL

10x CIP: 0.43μL

10x Buffer: 1.72μL


Total: 17.2μL


2. Assemble the following reaction in a sterile microcentrifuge tube:

vector DNA 100ng

insert DNA 33ng

2X Rapid Ligation Buffer 5μl

T4 DNA Ligase (Weiss units) 3u

Nuclease-Free Water to final volume of 10μl

2. Incubate the reaction at room temperature for 5 minutes for cohesive-ended ligations, or 15 minutes for blunt-ended ligations.rature for 5 minutes for cohesive-ended ligations, or 15 minutes for blunt-ended ligations.

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