Primers for Ganoderma lucidum

Name Sequence (5'->3') Comments

• pHg_LB_For(sequencing) TGGTGGCAGGATATATTGTGGTGTAAACA This is to amplify the T-DNA region in pHg (or any pCAMBIA-1300 based vector)
• pHg_RB_Rev(sequencing) ccgttattctaataaacgctcttttctcttaggtttac This is to amplify the T-DNA region in pHg (or any pCAMBIA-1300 based vector)

• pSILBAgamma_For(sequencing) ggatcctctagaaagaaggattacctctaaacaag For amplifying silencing cassette region from pSILBAgamma
• pSILBAgamma_Rev(sequencing) gaattcagaggtccgcaagtagattgaaag

• pHg_L.bicolor_internalSeq_F aaagcatcagctcatcgagagcc

• pSILBA_For_seq2 tacgccaagcgcgcaattaac
• pSILBA_Rev_seq2 caaggcgattaagttgggtaacgc

• psdhB_H2L_for aatgaatgaccaacaagaccgt This is to amplify the H->L mutation in the psdhB_GL_ plasmid
• psdhB_H2L_rev acacctacctctccgccat This is to amplify the H->L mutation in the psdhB_GL_ plasmid

• pHg_rev_sdhBp CTTGAGATATGAAAAAGCCTGAACTCACC no overhang
• pHg_for_sdhBp TTCTTCAGCTTGATATCGAATTCCTGC w/ 20bp OH for Gibson w/ sdhBp

• pHg_for_HyrgoR(Gano) atctcaagccatggcgataagcttg no OH
• pHg_rev_HygroR(gano) agtagatgccgaccggaagc no OH

• pHg_rev_GPDp cttgagatatgaaaaagcctgaactcacc no OH, can use pHg_rev_sdhBp
• pHg_for_GPDp TTCTTCAGCTTGATATCGAATTCCTGCAG no OH, can use pHg_for_sdhBp

• pHg_forMid_HygroR(gano) AGCCATGACCCAGTCACGTAGC
• pHg_revMid_HygroR(gano) cgcatagttaagccagtatacactccg

• pHg_rev_hphSeq cgtctcgaatgttctcggtgtttagg
• pHg_for_hphSeq CACATGAGCGAAACCCTATAGGAACC

• pHg_for_SeqPrmtr AAACTCCCCAATGTCAAGCACTTCC
• pHg_rev_SeqPrmtr tcgtgccagctgcattaatgaatc
• F_G3PDp_105971SS1 AGAGGCGTCGTCAACG
• R_rfp_G3PDp_10597SS1 ACGTCCTCGCTGCTGGCCATCCGTTAATTCCGACTTTGACCTAG
• F_rfp_G3PDt_10597SS1 ACTCCGCCTCCGTTGACCGCGTAGAGGCAGATTGCAAGGTCTTG
• R_G3PDt_10597SS1 AATCGCGAGCTCACTAATCACCC
• R_RFP_ohG3PDt ACCTTGCAATCTGCCTCTACGCGGTCAACGGAGGC
• F_RFP_G3PDp GTCAAAGTCGGAATTAACGGATGGCCAGCAGCGAGG
• F_G3PDp_OHpHg GACGTTTTTAATGTACTGAAAGAGGCGTCGTCAACG
• R_G3PDp_OH_pHg_mRFP GCCGACCGATTCGGCGTTAAAATCGCGAGCTCACTAATCACCC
• F_pHg_OH_G3PDp_mRFP TGATTAGTGAGCTCGCGATTTTAACGCCGAATCGGTC
• R_pHg_OH_G3PDp_mRFP AGGGCGTTGACGACGCCTCTTTCAGTACATTAAAAACGTCCGC
• pshdB_1_midFor caggacgaaccgtttttcattaccg
• psdhB_1_midRev ctccgcctcgatctcttcgg
• seq_midbkbn_0001 gcttaactatgcggcatcagagc
• seq_pro_0001 gactgatgggctgcctgtatcg
• seq_RFP_0001 GCGACGGAGCTATTAGGACATGG
• seq_term_0001 GTGCGTGTAAAACTGAAAGACCGTG
• seq_bk2_0001 GCTTCGGGCCATATCAACGC
• psdhB_seqFor_sdhBp cagatcggtaccagccaaatgc

pHg(Ecoli)_G3PDp(GanoLuc)

• pHg_Rev_G3PDpGL GTCAAAGTCGGAATTAACGGATGAAAAAGCCTGAACTCACCGC
• Rev_G3PDp_pHgLB GTGAGTTCAGGCTTTTTCATCCGTTAATTCCGACTTTGACCTAGAAGTAC
• pHg_mRev_2020 GCAGCGACCGAAGGGTAGG
• pHg_mFor_2020 CAGCCGAAGAGCTGCAAAAAGC
• For_G3PDpGL_pHgLB TTCGATATCAAGCTGAAGAAAGAGGCGTCGTCAACGCC
• pHg_For_G3PDpGL AGGGCGTTGACGACGCCTCTTTCTTCAGCTTGATATCGAATTCCTGCAG

pHg_GL

• F_pHgGL_G3PDt TGATTAGTGAGCTCGCGATTTTCTTCAGCTTGATATCGAATTCCTGC
• R_G3PDt_pHgGL TTCGATATCAAGCTGAAGAAAATCGCGAGCTCACTAATCACCC
• F_G3PDt_hph GTCCCCGGGCTAAAAAGTGAGTAGAGGCAGATTGCAAGGTC
• R_hph_G3PDt ACCTTGCAATCTGCCTCTACTCACTTTTTAGCCCGGGGACG
• F_hph_G3PDp GTCAAAGTCGGAATTAACGGATGAAGAAGCCGGAGCTTAC
• R_G3PDp_hph GTAAGCTCCGGCTTCTTCATCCGTTAATTCCGACTTTGACCTAGAAG
• F_G3PDp_pHgGL GTACTGAATTAACGCCGAATAGAGGCGTCGTCAACG
• R_pHgGL_G3PDp AGGGCGTTGACGACGCCTCTATTCGGCGTTAATTCAGTACATTAAAAAC

pcbx_mRFP

• F_G3PDp_pCbxOH CAGCTATGACCATGATTACGAGAGGCGTCGTCAACG
• R_pCbx_G3PDpOH AGGGCGTTGACGACGCCTCTCGTAATCATGGTCATAGCTGTTTCCTGTG
• R_G3PDt_pCbxOH TGATCCAAGCTCAAGCTGCTAATCGCGAGCTCACTAATCACCC
• F_pCbx_G3PDtOH TGATTAGTGAGCTCGCGATTAGCAGCTTGAGCTTGGATCAGATTG
• hph_pHg_seq aggcaggtcttgcaacgtg hs
• Rseq_pHg CACATACAAATGGACGAACGGATAAACC rs
• cbxFor
• cbxRev
• R_g3pdt_popen CCACTTCTTCCCCGATAACGAATCGCGAGCTCACTAATCACCC
• F_g3pdp_popen ACCGATATGGCCAGTGTGCCAGAGGCGTCGTCAACG
• R_popen_g3pdp AGGGCGTTGACGACGCCTCTGGCACACTGGCCATATCGG
• popen_midFor CAACTTTATCCGCCTCCATCCAGTC
• F_popen_g3pdt TGATTAGTGAGCTCGCGATTCGTTATCGGGGAAGAAGTGGCTG
• popen_midRev CTGGCGAACTACTTACTCTAGCTTCC
• seqRev_G3PDt_2 GTATGTACCTCCGGTTTGCGTGATC

pHygR

• r_pHygR_G3PDt TGATTAGTGAGCTCGCGATTATTCGGCGTTAATTCAGTACATTAAAAAC
• f_pHygR_g3pdp AGGGCGTTGACGACGCCTCTTTCTTCAGCTTGATATCGAATTCCTGC
• f_g3pdp_pHygR TTCGATATCAAGCTGAAGAAAGAGGCGTCGTCAACGCC
• r_g3pdt_pHygR GTACTGAATTAACGCCGAATAATCGCGAGCTCACTAATCACCC

pCbxR(RP)_fuGFP

• f_fuGFP_g3pdp GTCAAAGTCGGAATTAACGGATGGTCAGCAGCGGGGAGG
• r_fugfp_g3pdt ACCTTGCAATCTGCCTCTACTCAGGACTTGTAAAGCTCGTCCATGC
• r_g3pdp_fugfp TCCTCCCCGCTGCTGACCATCCGTTAATTCCGACTTTGACCTAGAAG
• f_g3pdt_fugfp ACGAGCTTTACAAGTCCTGAGTAGAGGCAGATTGCAAGGTC

pOpen_Myco_HygR

• r_popen2_g3pdp AGGGCGTTGACGACGCCTCTGCGATGGCGAAGACTACTCCC
• f_popen2_g3pdt TGATTAGTGAGCTCGCGATTGCGATGTTGAAGACCACGCTGAG
• f_g3pdp2_popen GGAGTAGTCTTCGCCATCGCAGAGGCGTCGTCAACGC
• r_g3pdt2_popen AGCGTGGTCTTCAACATCGCAATCGCGAGCTCACTAATCACCC

pOpen_Myco_fuGFP

• mfor_popen GCTCATCATTGGAAAACGTTCTTCGG
• mrev_popen GTTACATCGAACTGGATCTCAACAGCG

pOpen_Myco_NanoLuc

• r_g3pdp_nanoluc TCTTCGAGTGTGAAGACCATCCGTTAATTCCGACTTTGACCTAGAAG
• f_g3pdt_nanoluc GCGAACGCATTCTGGCGTAAGTAGAGGCAGATTGCAAGGTCTTG
• f_nanoluc_g3pdp GTCAAAGTCGGAATTAACGGATGGTCTTCACACTCGAAGATTTCG
• r_nanoluc_g3pdt ACCTTGCAATCTGCCTCTACTTACGCCAGAATGCGTTCGC
• F_pOM_HygR ggctacggtctcccttcttgagaagaactagcataaccccttggggcctc
• R_pOM_HygR ggctacggtctcattgcgtttgtcctcagcggctccgacgattgacac
• F_TEF1t ggctacggtctcagcaaagaaacccccgggagtag
• R_TEF1t ggctacggtctctagtcctgaggcccactgagcatgatctgatc
• F_fuGFP_pOMHygR ggctacggtctctgacttgtaaagctcgtccatgcc
• R_fuGFP_pOMHygR ggctacggtctcttggtcagcagcggggaggatat
• F_TEF1p ggctacggtctctaccatcatggtcctcagggctgagatc
• R_TEF1p ggctacggtctccgaagcacttccgaatttgcattatcag