PMSF

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[edit] Purpose

PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations.

Molecular W.: 174.19

[edit] Procurement

You can buy PMSF for example at Pierce or Sigma.

[edit] Use

Don't try solution in water, it does not work. Take ethanol or methanol. 200 mM stock solution are stable for months at 4°C. A normal endconcentration of PMSF is probably about 1mM of course depending on the experimental setup.

[edit] Safety

PMSF is poisenes and should be handeld carefully.