Nachury:Protocols
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Under construction
Cell Lines
Establishing stable lines with retrovirus
Cloning fluorescent cells by FACS
Growing and harvesting cells in large amounts
Extractology
Hypotonic/N
2
cavitation lysis
Testis extract
Retinal extract
Retinal detergent extract
Protein Biochemistry
TAP purification of protein complexes from mammalian cells
Silver staining
Methanol-Chloroform precipitation of proteins
TCA precipitation of proteins
Immunofluorescence
General guidelines
PFA/Saponin
Lipids
Preparation of liposomes
Protein-Liposomes interaction assays
PIP strip blotting assays (fat blot)
DNA
Old-school maxiprep
Microtubules
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