Muratore:Protocols/Digest/SapI
From OpenWetWare
(Redirected from Muratore:Protocol/Digest/SapI)
| Tube | sterile H2O | 10X NEB4 | DNA | SapI (2 kU/mL) |
|---|---|---|---|---|
| plasmid | 30 μL | 5 μL | 10 μL of NheI digest | 5μL |
| insert | 30 μL | 5 μL | 10 μL of NheI digest | 5μL |
- 2h @ 37°C
- spin-column purify
