Moore Notes 10 16 13
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Group Call
- Participants: Katie, Stephen, Guillaume, Sarah, Ladan, Tom, Josh
- Discussion of Jack Gilbert's work on PICRUSTS and niche modeling
- PICRUSTs/MAP vs. shotgun metagenomes
- Their previous analysis of English Channel data found high correlation
- They used an old version of PICRUSTs plus their own pipeline
- Differentiating our projects
- Different scientific questions (changes in ecosystem services)
- They are processing EMP data sets, we would process MICROBIS
- PICRUSTs/MAP vs. shotgun metagenomes
- Jack joined our call
- Paper on predicting carbon flux in English Channel has been hard to publish
- Concern about over extrapolating or over-fitting models or over-interpreting features in the data vs. directly measuring ecosystem services
- Same techniques on Gulf of Mexico data
- What other things Jack wants to do with niche modeling in future vs. us?
- Jack: metabolic prediction, stability issues with key biogeochemical cycles associated with human activity (spatial and temporal activity of pollution events) using MAP/PMRT
- Josh: functional diversity, survey gap analysis, extinctions
- Doesn't want us to reinvent the wheel with English Channel data
- They are doing functional predictions from EMP data, not MICROBIS
- Could compare EMP vs. MICROBIS maps in a paper about sampling issues, reliability of methods, etc.
- EMP primers are not good at picking up SAR11 (Furman noticed this)
- Tara Oceans coming out soon
- Will see where they sampled, then use EMP or call to community to fill gaps (e.g., based on conclusions of EMP vs. MICROBIS maps)
- Josh: Do you know any one sampling marine hosts? Whales, sponges
- Jack has looked at boats, also microbes on plants (merlot vines)
- American gut, mouse guts around N. America - correlated?
- Jack talked about running MAP on American gut, but metadata is challenging
- 5-6K people with data from phones and shoes - this would be good for niche modeling
- Tibet soil project might be of interest to Nature
- Gulf of Mexico raster data
- Paper on predicting carbon flux in English Channel has been hard to publish