McClean: Phosphate Buffered Saline
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Materials
- sodium chloride (NaCl)
- potassium chloride (KCl)
- sodium phosphate, dibasic (Na2HPO4)
- potassium phosphate, monobasic (KH2PO4)
Protocol
To make 1L of a 10x solution:
- Add together:
- 80 g sodium chloride (NaCl)
- 2 g potassium chloride (KCl)
- EITHER 14.4 g of anhydrous sodium phophate, dibasic (Na2HPO4) OR 14.4g (anhydrous) x 268.07 g/mol (heptahydrate) / 141.96 g/mol (anhydrous) = 27.2 g of heptahydrate sodium phophate, dibasic
- 2.4 g potassium phosphate, monobasic (KH2PO4)
- Adjust pH to 7.4 with HCl if necessary.
- Bring to 1 L with dH2O.
- Autoclave