McClean: Phosphate Buffered Saline

From OpenWetWare

Jump to: navigation, search

Materials

  • sodium chloride (NaCl)
  • potassium chloride (KCl)
  • sodium phosphate, dibasic (Na2HPO4)
  • potassium phosphate, monobasic (KH2PO4)

Protocol

To make 1L of a 10x solution:

  • Add together:
    • 80 g sodium chloride (NaCl)
    • 2 g potassium chloride (KCl)
    • 14.4 g sodium phophate, dibasic (Na2HPO4)
    • 2.4 g potassium phosphate, monobasic (KH2PO4)
  • Adjust pH to 7.4 with HCl if necessary.
  • Bring to 1 L with dH2O.
  • Autoclave
Personal tools