McClean: Phosphate Buffered Saline

Materials

• sodium chloride (NaCl)
• potassium chloride (KCl)
• sodium phosphate, dibasic (Na₂HPO₄)
• potassium phosphate, monobasic (KH₂PO₄)

Protocol

To make 1L of a 10x solution:

• Add together:
  • 80 g sodium chloride (NaCl)
  • 2 g potassium chloride (KCl)
  • EITHER 14.4 g of anhydrous sodium phophate, dibasic (Na₂HPO₄) OR 14.4g (anhydrous) x 268.07 g/mol (heptahydrate) / 141.96 g/mol (anhydrous) = 27.2 g of heptahydrate sodium phophate, dibasic
  • 2.4 g potassium phosphate, monobasic (KH₂PO₄)
• Adjust pH to 7.4 with HCl if necessary.
• Bring to 1 L with dH2O.
• Autoclave