McClean: Glucose Solution

Overview

Use this protocol to make 20% or 40% glucose for adding to media recipes.

Materials

1. Dextrose
2. Mili-Q Water

Procedure

To make 20% (10x concentration of what yeast normally need) glucose

• Weight out 20g of glucose (dextrose)
• Add to 70ml of H₂O
• Dissolve by stirring. May use some heat.
• Once the sugar has dissolved, bring the volume of the mix up to 100ml total.
• Autoclave.

To make 40% (20x concentration of what yeast normally need) glucose

• Weight out 40g of glucose (dextrose)
• Add to 70ml of H₂O
• Dissolve by stirring. May use some heat.
• Once the sugar has dissolved, bring the volume of the mix up to 100ml total.
• Autoclave.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. List troubleshooting tips here.
2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
3. Anecdotal observations that might be of use to others can also be posted here.

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Contact

• Megan N McClean 14:01, 20 July 2011 (EDT)

or instead, discuss this protocol.