McClean: FISH ConA
Overview
This is the concanavalin A solution we use for FISH and for microscopy applications where the small amount of ions in the "microfluidics" conA might be problematic.
Materials
- Concanavalin A (MP Biomedicals, cat no: 150710)
- Sterile H2O
Procedure
Dissolve a 20mg/ml solution of conA in sterile water. Dilute from this mixture down to 2mg/ml in sterile miliQ water. Store the 2mg/ml aliquots at -20°C, thaw just before microscopy or FISH. Concanavalin A solution can be stored at room temperature for several days without losing efficiency.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
*Megan N McClean For FISH you will need to dilute this solution further (1:20 dilution in sterile water to 0.1mg/ml concentration)
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
Contact
- Megan N McClean 14:01, 23 September 2011 (EDT)
or instead, discuss this protocol.
