McClean: FISH ConA
This is the concanavalin A solution we use for FISH and for microscopy applications where the small amount of ions in the "microfluidics" conA might be problematic.
- Concanavalin A (MP Biomedicals, cat no: 150710)
- Sterile H2O
Dissolve a 20mg/ml solution of conA in sterile water. Dilute from this mixture down to 2mg/ml in sterile miliQ water. Store the 2mg/ml aliquots at -20°C, thaw just before microscopy or FISH. Concanavalin A solution can be stored at room temperature for several days without losing efficiency.
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
*Megan N McClean For FISH you will need to dilute this solution further (1:20 dilution in sterile water to 0.1mg/ml concentration)
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
- Megan N McClean 14:01, 23 September 2011 (EDT)
or instead, discuss this protocol.