McClean:LB1

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Contents

Overview

LB agar media

Reagents

  • Bacto tryptone
  • Bacto yeast extract
  • NaCl
  • Bacto Agar

Protocol

  • Begin with approximately 800mls of MiliQ water
  • Weigh out and add ingredients with constant stirring:
    • Bacto tryptone 10g
    • Bacto yeast extract 5g
    • NaCl 5g
    • Bacto Agar 15g
  • Bring up to 1000mls with MiliQ water
  • Separate into two 1L bottoles of 500mls each
  • Autoclave


Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.


*Megan N McClean 13:24, 2 July 2015 (EDT) Many LB formulations call for twice as much NaCl (10g instead of 5g in this protocol).

References

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


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