McClean:General Flow Cytometry Procedure

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Materials

  1. PBS + 0.1% (vol/vol) Tween 20
  2. Falcon 2058 6mL tubes
  3. a sonicator
  4. BD LSRII

Procedure

  1. For each sample fill a falcon tube with 0.8mL of PBS + 0.1% Tween and put it on ice
  2. Grow yeast up and run experiment
  3. When you want to take a sample pipet 250 μL of culture into the corresponding falcon tube
  4. Sonicate the cells with ten 0.5 second long bursts.
  5. Measure fluorescence with the BD LSRII
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