McClean:General Flow Cytometry Procedure
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Materials
- PBS + 0.1% (vol/vol) Tween 20
- Falcon 2058 6mL tubes
- a sonicator
- BD LSRII
Procedure
- For each sample fill a falcon tube with 0.8mL of PBS + 0.1% Tween and put it on ice
- Grow yeast up and run experiment
- When you want to take a sample pipet 250 μL of culture into the corresponding falcon tube
- Sonicate the cells with ten 0.5 second long bursts.
- Measure fluorescence with the BD LSRII