McClean:5FOA

5-FOA Plates

For 500ml:

• 5 ml 100x Zymo Research liquid 5-FOA
• 150ml 3.33x YNB w/o amino acids
• 50ml 20% glucose
• 50ml KS-URA (10x) amino acid solution
• 250ml 2x bacto agar
• 25mg uracil

Microwave the bacto agar to re-liquify. Allow to cool to the touch but not to solidify. Add the rest of the components (protocols can be found under media for the basic items such as YNB w/o amino acidS) to the bacto-agar. Swirl the bottom to mix the components well. If the agar is not mixing, microwave the media for short periods (30s) until everything mixes well.

Note: can also use KS (10x), which is 10 mg/500 mL for uracil, in which case you'd add another 15 mg of uracil to get the same molarity. (2024-03-04 Kevin Stindt)