Matt Gethers/CRI, Thailand/Specifications/pKn001/42.SEQ
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42.SEQ (pKn001.P1, pUC18_rev)
Base Number (Sequence File Annotation) = Contig Sequence File Annotation Number (No trimmed 5' bases)
Base Number (Vector NTI File Annotation) = 434 + Contig Sequence File Annotation Number
| Base Number (Sequence File Annotation) | Base Number (Vector NTI File Annotation) | Theoretical Base | Read Base | Notes |
| I'm ignoring through base 34: noisy reading. | ||||
| 240 | 674 | C | Space | Should be another C - good |
| 386 | 820 | G | Space | Should be a G - good |
| 388 | 822 | G | Space | Should be a G - good |
| 400 | 834 | G | Space | Could be a G - good |
| 413 | 847 | G | Space | Should be a G - good |
| 441 | 875 | G | Space | Should be a G - good |
| 456 | 890 | G | Space | Maybe another G, but not sure |
| 463 | 897 | G | Space | Should be another G - good |
| 472 | 906 | G | Space | Should be a G - good |
| 483 | 917 | G | Space | Doesn't look like there's another G - problem |
Notes
Region of Interest: Vector NTI annotation 437 - 1462
Looks good overall - just the last G/Space concerns me.
