Matt Gethers/CRI, Thailand/Specifications/pIs001/31.SEQ
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31.SEQ (pIs001.P1, T7_for)
PIs001contig.cep - Old alignment; annotations do not refer to this file.
Base Number (Sequence File Annotation) = Contig Sequence File Annotation Number + 29 where 29 is the number of trimmed 5' bases.
Base Number (Vector NTI File Annotation) = 5310 + Contig Sequence File Annotation Number
| Base Number (Sequence File Annotation) | Base Number (Vector NTI File Annotation) | Theoretical Base | Read Base | Notes |
| 39 | 5320 | G | N | Looks like a G - good |
| 40 | 5321 | G | N | Looks like a G - good |
| 78 | 5359 | G | T | Looks like a T - problem! |
| 81 | 5361 | A | C | Could be another A - good |
| 82 | 5262 | G | C | Could be a G - good |
| 83 | 5263 | G | N | Looks like a G - good |
| 369 | 5650 | C | Space | Could be a second C - good |
| 391 | 5672 | Space | G | Insertion of G - problem! Absent the G, sequence from 390 should be CAACCGTGG |
| 394 | 5675 | Space | C | Could be another C - good |
| 414 | 5695 | G | N | Could be a G - good |
| 424 | 5705 | G | Space | Could be a G - good |
| 448 | 5729 | C | A | Could be a C - good |
| 452 | 5733 | G | A | Could be a G - good |
| 464 | 5745 | T | N | Looks like a T - good |
Notes
Region of Interest (Vector NTI Annotation): 5318 - 6121 (ATG to TGA)
