Matt Gethers/CRI, Thailand/Labwork/Phosphatase Treatment
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Alkaline Phosphatase Treatment (SAP Enzyme)
| Reagent | Volume | Notes |
| Digested Vector | 8.5 μl | |
| SAP Buffer | 1 μl | |
| SAP Enzyme | 0.5 μl | |
| Total | 10 μl |
Incubate mix at 37oC for 30 minutes, then inactivate at 65oC for 15 minutes.
Run Notes
7.22.08
Ran protocol as written to dephosphorylate pKn002.P1 cut with SphI and HindIII.
